特应性皮炎患者外周血皮肤归巢的CD8＋T细胞研究  

Peripheral blood skin-homing CD8＋ T cells in patients with atopic dermatitis

作　　者：张宝祥[1] 林茂[2] 段志武 张佃财[1] 

机构地区：[1]山东省潍坊市益都中心医院皮肤性病科,262500 [2]大连医科大学附属第二医院皮肤性病科

出　　处：《中华皮肤科杂志》2014年第1期19-21,共3页Chinese Journal of Dermatology

摘　　要：目的探讨特应性皮炎（AD）患者外周血CD8＋T细胞皮肤归巢及杀伤功能相关蛋白的表达。方法15例AD患者和14例健康人，用流式细胞仪，检测外周血CD8＋T细胞、表达皮肤淋巴细胞相关抗原的CD8＋T细胞（CLA＋CD8＋T细胞）的比例。结果CD8＋T细胞比例在AD组和对照组之间差异无统计学意义（P〉0．05）；CD8＋T细胞穿孔素、颗粒酶B和FasL的表达在两组间差异亦无统计学意义（均P〉0．05oCLA＋CD8＋T细胞比例在AD组（3．80％±1．46％）高于健康对照组（2．18％±0．85％）（t=3．636，P〈0．叭），AD组CLA＋CD8＋T细胞比例与sc0RAD（SCORingofAtopicDermatitis）评分呈正相关（r=0．565，P〈0．05）；CCR4在CD8‘T细胞表达在AD组（13．86％±4．42％）高于健康对照组（9．50％±2．14％）（t=3．738，P〈0．01），而CCR10和CXCR6的表达在两组间差异均无统计学意义（P〉0．05）.CLA＋CD8＋T细胞穿孔素的表达在AD组（74．27％±15．94％）高于健康对照组（57．20％±14．64％）（t=2．998，P〈0．01），颗粒酶B的表达在AD组（70．90％-4-13．85％）也高于健康对照组（56．41％±11．00％）（t=3．104，P〈0．01），而FasL的表达在两组间差异无统计学意义（P〉0．05）.CLA＋CD8＋T细胞CCR4、CCR10和CXCR6的表达在AD组与对照组间差异无统计学意义（均P〉0．05o结论AD患者外周血CLA＋CD8＋T细胞数量增加，杀伤功能相关蛋白穿孔素、颗粒酶B的表达增强，可能参与了AD的发病。Objective To quantify the percentage of CD8 ~ T cells and their expressions of cytotoxic molecules and homing-related chemokine receptors in peripheral blood from patients with atopic dermatitis （AD）. Methods Peripheral blood was obtained from 15 patients with AD and 14 healthy controls. Flow cytometric analysis was performed to determine the percentages of CD8＋ T cells and CD8＋CLA＋ T cells in the peripheral blood samples, as well as the expression levels of cytotoxic molecules and homing-related chemokine receptors on these cells. Differences in these parameters were analyzed using t test, and relationship between these parameters was evaluated using Pearson correlation coefficient. Results No significant difference was observed between the patients with AD and healthy controls in the percentage of CD8＋ T cells, the expressions of perforin, granzyme B, CCR10, CCR6 or FasL on CD8＋ T cells, or the expressions of CCR4, CCR10, CXCR6 or FasL on CLA＋CD8＋ T cells （all P 〉 0.05）. A significant increase was noted in the percentage of CLA＋CD8＋ T cells （3.80% ＋ 1.46% vs. 2.18% ＋ 0.85%, t = 3.636, P 〈 0.01 ） and expression rates of CCR4 on CD8＋ T cells （13.86% ± 4.42% vs. 9.50% ± 2.14%, t = 3.738, P 〈 0.01） as well as perforin and granzyme B on CLA＋CD8＋ T cells （74.27% ＋ 15.94% vs. 57.20% ± 14.64%, t = 2.998, P〈 0.01; 70.90% ± 13.85% vs. 56.41%± 11.00%, t = 3.104, P〈 0.01） in the patients with AD compared with the healthy controls. Conclusions The proportion of CLA＋CD8＋ T cells is increased with enhanced expressions of cytotoxie molecules such as perforin and granzyme B in peripheral blood of patients with AD, which may contribute to the pathogenesis of AD.

关 键 词：皮炎 特应性 CD8阳性T淋巴细胞 皮肤淋巴细胞相关抗原 颗粒酶类 受体 趋化因子 

分 类 号：R758.2[医药卫生—皮肤病学与性病学]