慢性特发性荨麻疹患者相关转录因子FOXP3、RORγt基因的表达失衡  

Imbalanced expression of transcription factors FOXP3 and RORγt in patients with chronic spontaneousurticaria

作　　者：朱慧兰[1] 杨婧[1] 李润祥[1] 梁碧华[1] 梁艳华[1] 毕超[1] 

机构地区：[1]广州市皮肤病防治所,510095

出　　处：《中华皮肤科杂志》2014年第1期53-54,共2页Chinese Journal of Dermatology

基　　金：基金项目：广东省自然科学基金（S2012020011077）

摘　　要：目的探讨Treg／Th17细胞在慢性特发性荨麻疹发病机制中的作用。方法89例CSU患者分为活动期组48例，静止期组41例，同期我市社区医院健康体检者48例作为对照组。用荧光定量PCR检测检测研究对象外周血单一核细胞（PBMC）中FOXP3、RORTtmRNA的表达水平。结果活动期组PBMCFOXP3mRNA表达水平（0．57±0．19）显著高于静止期组（0．11±0．21）和健康对照组（0．13±0．23），差异有统计学意义（P〈0．05）。活动期组PBMCROγtmRNA表达水平（0．43±0．39）显著低于健康对照组（0．87±0．43）和静止期组（0．89±0．40），差异有统计学意义（P〈0．05）。结论调控Treg细胞的相关转录因子FOXP3表达水平升高，调控Th17细胞的相关转录因子RORγt表达降低，两种基因的相互作用导致Treg／Th17细胞失衡可能是CSU发生的原因之一。Objective To investigate the role of regulatory T （Treg） / T helper type 17 （Th17） cells in the pathogenesis of chronic spontaneous urticaria （CSU）. Methods Eighty-nine patients with CSU were enrolled in this study, including 48 in active stage and 41 in remission stage. Forty-eight health check-up examinees, who were collected from the community hospitals in Guangzhou city, served as the healthy controls. Fluorescence-based real- time quantitative PCR was performed to determine the expression of transcription factors FOXP3 and RORγt in PBMCs from these subjects. Results Compared with the patients with CSU in remission stage and healthy controls, the patients in active stage showed a significantly higher level of FOXP3 mRNA （ 0.57± 0.19 vs. 0.11 ± 0.21 and 0.13 ± 0.23, both P 〈 0.05）, but a significantly lower level of RORγt mRNA （0.43 ± 0.39 vs. 0.89 ± 0.40 and 0.87 ± 0.43, both P 〈 0.05）. Conclusions The expression of Treg cell regulator FOXP3 increases, while the expression of Thl7 cell regulator RORγt decreases in patients with CSU, suggesting that the imbalance between Treg and Thl7 ceils induced by the interaction between FOXP3 and RORγt may be involved in the pathogenesis of CSU.

关 键 词：荨麻疹 T淋巴细胞 调节性 Th17细胞 转录因子 

分 类 号：R758.24[医药卫生—皮肤病学与性病学]