人类辅助生殖技术中胚胎质量评估方法的研究进展  

Research Progress of Embryo Quality Evaluation in Human Assisted Reproductive Technology

作　　者：赵杰[1] 陈秀娟[1] 何江峰[2] 

机构地区：[1]内蒙古医科大学附属医院妇产科生殖中心,呼和浩特010050 [2]内蒙古农牧业科学院生物技术研究中心,呼和浩特010031

出　　处：《生殖与避孕》2014年第1期65-72,共8页Reproduction and Contraception

摘　　要：胚胎质量评估方法的优劣直接关系到辅助生殖临床妊娠结局的好坏。目前,对胚胎质量评估的方法中,应用最广泛的是对配子、胚胎各时期的形态特征进行评分的形态学评价法;近来,在形态学评分系统的基础上,结合实时成像分析系统对胚胎发育过程进行连续观察并对胚胎进行选择,其应用价值已得到确认。而通过测定卵泡液及培养基中代谢物的代谢组学法虽然能更有效地分析胚胎活性、预测临床结局,但因受检测技术手段的限制,该方法尚未广泛应用于临床。且形态学和代谢组学分析不能确定染色体的异常,因此,染色体筛查已成为选择活力胚胎的方法之一,但是该法会对胚胎造成损伤及结果偏差较大,其应用还需要进一步评估。Optimal or slight method on embryo quality evaluation is directly related to the clinical pregnancy outcome of assisted reproduction. Currently, in the embryo quality evaluation, the most widely used method is morphological evaluation method which is grading the morphological characteristics of gamete and embryo in each period. Recently, the embryo selection accords to continuous observation of the embryonic development process by morphological grading system combined with the time-lapse imaging analysis system, which application value has been recognized. The metabonomics method according to determination of the metabolites in follicular fluid and culture medium is more efficient than others on the activity analysis of embryo and predication of clinical outcome. Nevertheless,it is not widely used in the clinic due to the limitation of detection technology. Morphological and metabonomics analysis can not determine whether the chromosomal is normal or not, consquently, the chromosome screeing has been one of methods in selecting viable embryo. Its application needs to be further evaluated because the method has large damage on embryo and the result deviation is more large.

关 键 词：胚胎发育 代谢组学 基因组学 植入前非整倍体筛查 

分 类 号：R714.8[医药卫生—妇产科学]