实验性自身免疫性脑脊髓炎发病过程中淋巴细胞相关因子及转录因子的表达  

Expressions of lymphocyte-associated cytokines and transcription factors during onset of experimental autoimmune encephalomyelitis

作　　者：刘宇[1] 杨金凤[1] 詹晓霞[1] 王心悦[1] 孙博[1] 

机构地区：[1]哈尔滨医科大学神经生物学教研室黑龙江省高校神经生物学重点实验室,黑龙江哈尔滨150081

出　　处：《中国生物制品学杂志》2014年第1期36-41,共6页Chinese Journal of Biologicals

基　　金：哈尔滨市科技局基金(2009RFXXS009;2008RFQXS085);国家青年自然科学基金(30901330;81000512;81000511)

摘　　要：目的探讨实验性自身免疫性脑脊髓炎(experimental autoimmune encephalomyelitis,EAE)发病过程中淋巴细胞相关因子及转录因子的表达情况。方法将C56BL/6小鼠分为免疫髓鞘少突胶质细胞糖蛋白35-55(myelin oligodendrocyte glycoprotein 35-55,MOG35-55)特异性免疫乳剂的EAE组与免疫未加MOG35-55多肽的免疫乳剂的CFA组,每组16只,分别取100μl免疫乳剂,经腋窝皮下免疫小鼠,于免疫当天及第2天经小鼠尾静脉注射200 ng百日咳毒素(pertussis toxin,PT),建立实验动物模型,并于免疫后进行临床评分;分别于免疫后第7、14和21天取小鼠的淋巴结和脾脏,RT-PCR法检测淋巴细胞相关因子及转录因子IL-17、IFNγ、RAR相关孤核受体γ(RAR-related orphan receptor gamma,RORγ)和T盒子转录因子be(tT-box transcription factor bet,T-bet)基因mRNA转录水平的变化。结果成功建立EAE模型,从免疫后第7天起出现临床症状,EAE组临床评分明显高于CFA组(P均<0.05);免疫后第7、14和21天,EAE组淋巴结和脾脏淋巴细胞中IL-17、IFNγ、RORγ、T-bet基因mRNA转录水平均明显高于CFA组。结论 IL-17、IFNγ、RORγ和T-bet参与EAE疾病的发生发展,且表达量的增加与疾病加重有相关性。Objective To investigate the expressions of lymphocyte-associated eytokines and transcription factors during onset of experimental autoimmune encephalomyelitis （EAE）. Methods EAE model was established by immunizing MOG33-35 （myelin oligodendrocyte glycoprotein 35-55） with CFA （complete Freunds adjuvant） subcutaneously into the axillae of sixteen female C57BL/6 mice, using CFA as control. The model mice were injected i. v. 200 ng pertussis toxin （PT） on days 0 and 2 after immunization, and recorded for clinical scores. The lymph nodes and spleens of mice were collected on days 7, 14 and 21 after immunization, and determined for the transcription levels of IL-17, IFNγ, RORγ （RAR-related orphan receptor gamma） and T-bet （T-box transcription factor bet） mRNAs. Results EAE model was sue- cessfully established. Clinical symptoms were observed starting from day 7 after immunization, and the clinical scores of mice in EAE group were significantly higher than those in CFA group （P 〈 O. 05）. The transcription levels of IL-17, IFNγ, RORγ and T-bet mRNAs in lymph nodes and spleens of mice in EAE group were significantly higher than those in CFA group. Conclusion IL-17, IFNγ, RORγ and T-bet participated in onset and progress of EAE, of which the ex- pression levels were related to the exacerbation of the disease.

关 键 词：实验性自身免疫性脑脊髓炎 淋巴细胞 白介素-17 干扰素Γ RAR相关孤核受体γ T盒子转录因子bet 

分 类 号：R593.2[医药卫生—内科学] R392.11[医药卫生—临床医学]