高效液相色谱-紫外检测法同时测定染发剂中的20种染料成分  

HPLC-DAD for simultaneous determination of 20 colorants in hair dyes

作　　者：钱跹 金柔男[1] 赵亮[1] 吕磊[1] 张海[1] 李悦悦[1] 张国庆[1] 

机构地区：[1]第二军医大学东方肝胆外科医院药材科,上海200438

出　　处：《第二军医大学学报》2014年第1期110-114,共5页Academic Journal of Second Military Medical University

摘　　要：目的建立高效液相色谱（HPLC）-二极管阵列检测器（DAD）法测定染发剂中20种染料成分的含量，对《化妆品卫生规范》中的氧化型染料的HPLC检测方法进行改进和补充。方法样品经乙醇一水超声提取，过滤后进样检测。色谱柱：Agilent Zorbax Bonus-RP（4．6mm×250mm，5μm）；流动相：A相为乙腈，B相为4mmol／L磷酸盐缓冲液（pH6．0，含0．1％的辛烷磺酸钠离子对试剂），梯度洗脱：0～24min，10Y0A；24～26min，10％～40％A；26～45min，40％A；45～47min，40％～80Y0A；47～52min，保持80％A不变；流速1mL／min；柱温15℃，使用DAD进行检测，检测波长为280nm，进样量5μL。结果各染料成分在50min内基线分离。在10．0～500．0mg／L浓度范围内，线性关系良好，相关系数均高于0．999。日内及日间精密度均小于4％，加样回收率为81．90％～119．88％。结论该方法简便、快速、准确，适于氧化型染发剂中染料成分的检测。Objective To develop a high-performance liquid chromatography （HPLC）-diode array detector （DAD） method for determination of 20 colorants in hair dyes, so as to improve the method for determining oxidative hair dyes in Hygienic Standard for Cosmetics. Methods After ultrasonic extraction with ethanol-water, samples were analyzed by HPLC. The separation was achieved on an Agilent Zorbax Bonus-RP column （4.6 mm×250mm,5μm） using a gradient mobile phase system of A： aeetonitrile and B： 4 mmol/L PBS （pH= 6.0） containing 0. 1%1-oetanesulfonic acid sodium salt solution at a flow rate of 1 mL/min. The gradient elution program was as follows： 0-24 rain, 10% A; 24-26 min,10%-40% A; 26-45 rain, 40% A; 45-47 min, 40%-80% A; 47-52 min, keep 80% A unchanged. The detection wavelength was set at 280 nm, the temperature of column was 15℃, and the injection volume was 5 μL. Results All the components were separated at baseline within 50 min. Within the concentration range of 10.0-500.0 mg/L, the peak areas and concentrations of colorants had good linear relationship, with the linear correlation coefficients being higher than 0. 999. The intra day and inter-day precisions were within 4% and the average recoveries ranged from 81. 90% to 119. 88%. Conclusion The present method is simple, rapid and accurate and is suitable for determination of colorants in oxidative hair dyes.

关 键 词：高压效液相色谱法 染料 染发剂 

分 类 号：R917.7[医药卫生—药物分析学]