Ghrelin对放烧复合伤大鼠的救治作用  

Therapeutic effects of ghrelin on combined radiation and burn injury in rats

作　　者：刘聪[1] 李宏[1] 李蓉[1] 粟永萍[1] 

机构地区：[1]第三军医大学军事预防医学院全军复合伤研究所,创伤,烧伤与复合伤国家重点实验室,重庆市纳米医药工程技术研究中心,重庆400038

出　　处：《第三军医大学学报》2014年第3期213-216,共4页Journal of Third Military Medical University

基　　金："十二五"国家科技支撑计划项目(2013BAK03B05-02);军队"十二五"重大项目(AWS11C002-009)~~

摘　　要：目的探索Ghrelin对放烧复合伤大鼠是否具有救治作用,并对其产生救治作用的可能机制做初步研究。方法 120只清洁级雄性SD大鼠按随机数字表法分为4组:正常组(Con)、单纯Ghrelin处理组(GHR,即对正常大鼠腹腔注射Ghrelin)、放烧复合伤组(CRBI,5 Gy全身均匀照射+15%全身体表面积皮肤Ⅲ度烧伤)、Ghrelin治疗组(CRBI+GHR),GHR每天1次,连续5 d腹腔注射,剂量3.37×10-2mg/kg,每组30只。观察项目包括:①大鼠30 d存活率;②造血功能:7、14、21 d外周血象和第10天脾结节形成实验、脾脏指数、骨髓有核细胞计数;③最后1次给药后2、4、6 h血清中炎性介质TNF-α、IL-6,神经递质乙酰胆碱(ACH),肾上腺素(E)及皮质醇(Cor)的含量。结果 Ghrelin可显著提高CRBI动物存活率(约25%);与CRBI组相比可显著提高第7天外周血中WBC计数(P<0.05),第10天骨髓有核细胞计数亦明显高于CRBI组,但在脾结节及脾脏指数方面尚未观察到显著差异(P>0.05);与CRBI组相比,还可使伤后第4天血清中TNF-α及IL-6含量降低(P<0.05),乙酰胆碱总体含量降低(P<0.05),肾上腺素及皮质醇水平下降(P<0.05)。结论 Ghrelin对放烧复合伤大鼠具有一定的救治作用,其作用的发挥可能与其减轻造血功能抑制,下调促炎介质水平并调节植物神经系统的兴奋性有关。Objective To determine the effects of ghrelin on combined radiation and burn injury in rats and investigate the possible mechanism involved in its therapeutic effects. Methods A total of 120 male SD rats were randomly divided into 4 groups, control group （Con） , gbrlein treatment group （GHR, receiving intraperitonea injection of ghrelin）, combined radiation and burn injury group （CRBI, receiving total body 5 Gy ^60Co irradiation and full-thickness burn injury on 15% TBSA） , combined injury with ghrelin treatment group （CRBI ＋ GHR）. Ghrelin was given to the corresponding rats through intraperitoneal injection at a dose of 3.37 x 10-2mg/kg for 5 consecutive days. Survival rate for 30 d, hematopoietic function, peripheral hemogram of the 7th, 14th, and 21st days, spleen nodes forming experiment, spleen index and bone marrow nucleated cell counts （BMNC） at the 10th day, serum levels of TNF-α, IL-6, acetylcholine （ACH）, epinephrine （E） and cortisol （Cor） at the 2nd, 4th, and 6th hours after the final injection of ghrelin were measured. Results Ghrelin significantly improved the survival rate of combined radiation and burn injury rats （about 25% compared with CRBI group）. As to the hematopoietic function, the WBC count at the 7th day and BMNC at the 10th day were significantly elevated （P 〈 0.05）. However, the spleen node counts and spleen index don＇t indicate a significant difference （ P 〉 0.05 ）. Compared with CRBI group, ghrelin treatment significantly reduced the content of proinflammatory mediator TNF-α and IL-6, neurotransmitter Ach and E, and endocrine hormone Cor （P 〈 0.05 ） in the serum at the 4th day after last ghrelin injection. Conclusion Ghrelin has therapeutic effect on combined radiation and burn injury rats, which might be due to its alleviating the inhibition of hematopoietic function, down-regulating the pro-inflammatory mediators and regulating the excitability of autonomic nervous system.

关 键 词：放烧复合伤 GHRELIN 救治作用 

分 类 号：R644.05[医药卫生—外科学] R818.74[医药卫生—临床医学]