携带神经干细胞的肌基膜管在大鼠脊髓损伤修复中的应用  

Application of muscle basal lamina containing neural stem cells in repair of spinal cord injury in rats

作　　者：胡杰亮[1] 康学文[1] 汪静[1] 王拴科[1] 王翠芳[1] 马靖琳[1] 安丽萍[1] 

机构地区：[1]兰州大学第二医院骨科研究所,甘肃省骨关节疾病重点实验室,730000

出　　处：《中华创伤杂志》2014年第1期73-77,共5页Chinese Journal of Trauma

基　　金：甘肃省自然科学基金资助项目（2009GS03710）

摘　　要：目的观察携神经干细胞（neuralstemcells，NSCs）的肌基膜管对脊髓损伤的修复作用。方法选择同一窝别SD大鼠36只，制备脊髓半切损伤模型，按随机数字表法分为空白对照组（脊髓断端处仅做等渗盐水冲洗）、NSCs组（脊髓断端处注射NSCs）和NSCs＋肌基膜管组（脊髓断端处植入NSCs＋肌基膜管复合体），每组12只。4，8周后观察移植细胞的存活、迁移、肌基膜管与宿主的组织相容性，采用BBB评分法观察术后2，4，8周各时相点大鼠后肢运动功能恢复情况。结果NSCs＋肌基膜管组NSCs能够在断端正常生长，并向断端头尾处迁移且与周围脊髓组织融合，而NSCs组断端处NSCs存活数量较少且炎症细胞浸润明显。术后2周BBB评分各组比较差异无统计学意义，术后4，8周BBB评分NSCs＋肌基膜管组分别为7．92±1．00，11．38±1．51，空白对照组分别为3．82±0．75，3．71±0．76，NSCs组分别为6．25±1．06，8．25±1．83，NSCs＋肌基膜管组明显高于空白对照组和NSCs组（P〈0．05）。结论肌基膜管能够提供NSCs沿其管腔定向生长基础，同时能够促进宿主自身细胞沿管腔内的基质成分爬行，减少局部炎症反应。Objective To observe effect of muscle basal lamina containing neural stem cells （NSCs） in repair of spinal cord injury. Methods Thirty-six SD rats from the same nest were used in the study and spinal cord hemisection models were induced. The animals were classified to blank control group （clearance of the lesion edge only with isotonic saline）, NSCs group （transplantation of NSCs to the edge）, NSCs ＋ muscle basal lamina group （ transplantation of complex of NSCs and muscle basal lam- ina to the edge） according to random number table, with 12 rats per group. At weeks 4 and 8, survival and migration of the transplanted ceils and compatibility of muscle basal lamina with the host were de- tected. At weeks 2, 4, and 8, the hindlimb function was assayed using BBB scoring system. Results NSCs in NSCs ＋ muscle basal lamina group grew at the lesion edge, migrated to both sides of the edge, and integrated with peripheral tissues. Whereas, few NSCs survived at the lesion edge in NSCs group and inflammatory cell infiltration was notable. At week 2, there was no statistical difference of BBB score a- mong the three groups. At weeks 4 and 8, BBB score in NSCs ＋ muscle basal lamina group （7.92 ±1.00, 11.38 ± 1.51 ） was significantly higher than that in blank control group （3.82± 0.75, 3.71±0.76） and NSCs group （6.25 ± 1.06, 8.25 ± 1.83 ） （P 〈 0.05）. Conclusion Muscle basal lamina orients growth of NSCs along its lumen, facilitates migration of host cells to ground substance within its lu- men, and reduces local inflammatory reaction.

关 键 词：脊髓损伤 神经干细胞 移植 肌基膜管 

分 类 号：R651.2[医药卫生—外科学]