拉贝洛尔对创伤性脑损伤大鼠空肠通透性的影响  

Effect of labetalol on the intestinal permeability in rats with traumatic brain injury

作　　者：郎宇璜[1] 陈凤媛[1] 席晨辉[1] 

机构地区：[1]复旦大学附属上海市第五人民医院创伤急救中心,上海200240

出　　处：《创伤外科杂志》2014年第1期42-45,共4页Journal of Traumatic Surgery

基　　金：上海市闵行区自然科学基金资助(2012MHZ013)

摘　　要：目的探讨拉贝洛尔对创伤性脑损伤(TBI)大鼠空肠通透性的影响。方法 54只雄性Wister大鼠,随机分为对照组(n=18)、TBI组(n=18)、TBI+拉贝洛尔组(n=18);每组伤后3、6、12h时间点各分为3个亚组(n=6),参照Feeney’s方法建立大鼠TBI模型。在各时间点采用异硫氰酸荧光素葡聚糖(FITCDextran)行大鼠活体内空肠通透性评价,酶联免疫吸附剂测定法(ELISA)测定血浆肾上腺素、肠道肿瘤坏死因子-α(TNF-α)水平,光学显微镜下观察各组肠黏膜组织形态变化。结果 TBI组大鼠在建模后各时间点血浆肾上腺素水平、肠道TNF-α水平和肠道通透性较对照组明显增加(P<0.01),TBI+拉贝洛尔组大鼠血浆肾上腺素水平和肠道TNF-α水平在建模后各时间点较TBI组大鼠明显降低(P<0.01,P<0.05),建模后6、12h TBI+拉贝洛尔组大鼠的肠道通透性较TBI组降低(P<0.05)。结论拉贝洛尔能降低大鼠TBI后空肠通透性的增加。Objective To study the effect of labetalol on tumor necrosis factor - alpha （ TNF - ct ） and per meability in rats after traumatic brain injury （TB[）. Methods Fifty-four male Wister rats were randomly divided into three groups ： control group （ n = 18 ） , TBI group { n = l 8 ） , TBI ＋ labetalol group （ n = 18 ）. Each group was fur-ther divided into 3 hours ,6 hours and 12 hours subgroups （ n = 6/subgroup ）. The rat TBI model was established by referring to Feeney＇ s method. After the model development, animals underwent assays of vivo intestinal permeabili- ty,evaluation of plasma epinephrine and intestinal TNF-aby ELISA method at each time point. And the pathologi- cal changes of intestinal mucosa were observed under light microscope. Results The levels of plasma epinephrine, intestinal TNF-a and permeability at each time point in TBI group were significantly higher than those in control group { P 〈 0. 01 ）. In TBI ＋ labetalol group, the levels of plasma epinephrine and intestinal TNF-a at each time point were significantly lower than those in TBI group （ P 〈 0. 01, P 〈 0. 05 ）. At 6 and 12 hours after model establish- ment, the level of intestinal permeability in TBI ＋ labetalol group was significantly lower than that in TBI group （ P 〈 0. 05 }. Conclusion Labetalol can decrease the levels of intestinal TNF-a and lessen the increase of intestinal per- meability of rats in TBI model.

关 键 词：创伤 脑损伤 拉贝洛尔 肿瘤坏死因子-A 肠道通透性 

分 类 号：R651.1[医药卫生—外科学]