胸部撞击伤复合脂多糖致急性肺损伤大鼠模型的建立  

Establishment of a rat model of acute lung injury following chest impact injury combined with LPS challenge

作　　者：王海燕[1] 杜娟[1] 赵玉金[1] 李海胜[1] 杨策[1] 岳彩黎[1] 蒋建新[1] 

机构地区：[1]第三军医大学大坪医院野战外科研究所第四研究室,创伤,烧伤与复合伤国家重点实验室,重庆400042

出　　处：《创伤外科杂志》2014年第1期46-49,共4页Journal of Traumatic Surgery

基　　金：国家"973"项目子项目(2012CB518104);创伤;烧伤与复合伤国家重点实验室自主研究课题(SKLZZ200804)

摘　　要：目的建立SD(Sprague Dawley)大鼠经胸部撞击复合内毒素所致急性肺损伤(ALI)模型,为后续ALI的救治和发病机制研究提供支撑。方法采用BMI-Ⅲ型生物撞击机,对SD大鼠行右侧胸部准静态正面撞击,比较动物受撞击后的呼吸及伤后24h肺部大体伤情。确认25kPa为合适的驱动压力后,于撞击后即刻通过尾静脉分别注射10、15、20mg/kg 3种剂量脂多糖(LPS),观察伤后动物的死亡率及肺损伤病理情况。结果 250kPa所致肺损伤伤情中度,动物无死亡。撞击后即刻复合LPS的3种剂量中,20mg/kg可致动物48h死亡率为80%,10mg/kg则致动物48h死亡率仅20%,15mg/kg致动物48h死亡率为70%。250kPa+15mg/kg条件下的肺组织病理检测可见撞击区旁红细胞、炎细胞聚集,肺泡腔及肺泡间隔出现粉红色液体,肺泡间隔增厚等ALI典型病变。结论以250kPa驱动压力行右侧胸部撞击复合15mg/kg的LPS静脉注射,既保证伤情达到一定严重程度,又保证后续能得到有效的治疗观察结果,为最佳致伤条件。所建立的大鼠ALI动物模型操作简便,致伤参数可控,且重复性好,病变典型,可作为研究急性肺损伤较理想的实验动物模型。Objective To establish an animal model of acute lung injury （ALl ） following chest impact combined with lipopolysaccharide （LPS） challenge. Methods The lung injury of rats was caused by the BM1-biological impact device on right lung at quasi-static pattern. Using 250 kPa as a suitable driving pressure, the chest injured rats were injected with 10,15,20 mg/kg LPS via tail vein respectively. The outcome of the ALI rats was ob- served. Results The driving pressure of 250 kPa induced moderate lung injury, no animal died after experiment. The mortality of chest injury rats with 20mg/kg of LPS was 80% within 48 hours,70% with lSmg/kg of LPS, and 20% with 10mg/kg of LPS. We set up the ALI model by 250 kPa driving pressure combined with 15mg/kg LPS. The biopsy features of the lungs demonstrated typical pathological changes after ALI. Conclusion 250 kPa driving pressure combined with 15mg/kg LPS is an ideal injury condition for ensuring a successful injury model. The estab- lished model of ALl is relatively simple, easily controllable and highly repeatable, which can be used as a feasible model for the study of ALI.

关 键 词：胸部损伤 肺损伤 撞击伤 生物撞击机 大鼠 

分 类 号：R655[医药卫生—外科学]