巨噬细胞迁移抑制因子在小鼠脊髓损伤后的表达研究  

Expression and effects of macrophage migration inhibitory factor on spinal cord injury in mice

作　　者：赵喆[1] 姚远[2] 杜长国 邓其跃[1] 

机构地区：[1]第三军医大学基础部神经生物学教研室,神经生物学重庆市市级重点实验室 [2]第三军医大学新桥医院骨科 [3]解放军77100部队后勤部门诊部

出　　处：《局解手术学杂志》2014年第1期1-4,共4页Journal of Regional Anatomy and Operative Surgery

基　　金：国家自然科学基金项目(31201018);重庆市自然科学基金项目(2008BB5110)

摘　　要：目的探讨巨噬细胞迁移抑制因子（MIF）在小鼠脊髓损伤后的修复作用。方法选择C57BL／6小鼠制作重物砸伤的脊髓损伤模型，分为手术组和假手术对照组，损伤节段为T。～T…用免疫组织化学法显示MIF在损伤急性期（术后72h）的表达改变。用RT—PCR显示MIF与RhoA的mRNA水平改变；用免疫荧光双标显示小胶质细胞表达MIF和RhoA的情况。结果重物坠落致脊髓损伤后72h，MIF在损伤段脊髓中蛋白表达量增加；同时MIF的mRNA水平升高，且伴随RhoA的mRNA水平升高，手术组和假手术组之间的差异有统计学意义（P〈0．05）。结论重物坠落致脊髓损伤后的急性期，损伤局部活化的小胶质细胞中MIF表达增加；少突胶质和星形胶质样细胞中有MIF累积，神经元中MIF的水平变化不显著。同时，MIF的mRNA水平也出现上调，与RhoA的mRNA上调在时空上有一致性，提示MIF可能调节损伤局部微环境参与轴突再生抑制。Objective To study the effects of macrophage migration inhibitory factor （MIF） on spinal cord injury. Methods C57BL/6 mice with injury at T9 - Tj0 were taken as spinal cord injury （SCI） model, and they were divided into the operated group and the sham oper- ated group. Immunohistochemistry was used to detect expression changes of MIF during 72 hours after operation. Reverse transcriptase-poly- merase chain reaction （RT-PCR） was used to test changes of mRNA level. And double staining immunofluorescence techniques was used to show expressions of MIF and RhoA. Results Expression of MIF at acute stage （72 hours after injury） increased significantly. Results of RT- PCR showed that mRNA levels of MIF and RhoA both increased. There were statistical differences between the two groups （ P 〈 0.05 ）. Con- clusion In acute stage after SCI, MIF increased in the activated microglia. MIF accumulated in oligodendrocyte-like and astrocyte-like cells in injured spinal cord, which might contribute to inhibitory environment for regeneration. Moreover, mRNA level of MIF raised with the in- crease of RhoA mRNA level, which indicated the potential inhibition of MIF to axonal regeneration in CNS.

关 键 词：巨噬细胞迁移抑制因子 脊髓损伤 Ras同族基因家族成员A 

分 类 号：R651.2[医药卫生—外科学] Q25[医药卫生—临床医学]