LATS1-YAP信号通路对人皮肤成纤维细胞增殖及细胞外基质合成的调控  

LATS1-YAP pathway regulate proliferation of human skin fibroblast and synthesis of extracellular matrix

作　　者：卢昊[1] 刘婷[1] 陈宇[1] 毛彤春[1] 雷泽源[1] 樊东力[1] 

机构地区：[1]第三军医大学新桥医院整形外科,重庆400037

出　　处：《局解手术学杂志》2014年第1期27-29,33,共4页Journal of Regional Anatomy and Operative Surgery

摘　　要：目的探讨LATS1-YAP信号通路对人皮肤成纤维细胞活力及细胞外基质合成的调控作用。方法实验分为未干预组、LATS1 siRNA干预组和YAP siRNA干预组。构建LATS1 siRNA和YAP siRNA,LATS1 siRNA干预组利用LATS1 siRNA转染人皮肤成纤维细胞株HS27,YAP siRNA干预组利用YAP siRNA转染HS27。转染后48 h利用Western-blot观察LATS1、YAP和collagenⅠ的表达情况,应用MTT检测HS27细胞株的细胞活力情况。结果 LATS1 siRNA转染48 h后LATS1蛋白表达显著降低,YAP蛋白和collagenⅠ蛋白表达升高,细胞活力显著增高;YAP siRNA转染48 h后LATS1蛋白表达无变化,YAP蛋白和collagenⅠ蛋白表达降低,细胞活力显著降低。结论 LATS1-YAP信号通路可调控人皮肤成纤维细胞活力和细胞外基质的合成,可能成为皮肤创伤后修复以及阻止创伤后瘢痕形成提供潜在治疗靶点。Objective To study the proliferation of human skin fibroblast and synthesis of extracellular matrix which were regulated by LATS1-YAP pathway. Methods They were divided into three groups ： control groups, LATS1 siRNA intervention group and YAP siRNA treatment group. Using LATS1 siRNA transferred human skin fibroblasts cell lines HS27 in LATS1 siRNA intervention group, and using YAP siRNA transferred HS27 in YAP siRNA treatment group. Expression of LATS1 ,YAP and collage I were detected by western-blot 48 h later, and the activity of HS27 cells was determined by MTI＇. Results Compared with control group,expression of LATS1 protein decreased while expression of YAP protein and collagen I protein increased 48 h after LATS1 siRNA transfection. Expression of LATS1 protein remains un- changed and expression of YAP protein and collagen I protein decreased 48 h after YAP siRNA transfection. Conclusion LATS1-YAP pathway could regulate proliferation of human skin fibroblast and synthesis of extracellular matrix. It provides a potential therapeutic targets for skin wound repair and cicatrization.

关 键 词：皮肤成纤维细胞 LATS1-YAP信号通路 增殖 细胞外基质 

分 类 号：R329.28[医药卫生—人体解剖和组织胚胎学] R619.6[医药卫生—基础医学]