小口径组织工程血管基质材料的生物学和力学评价  

Biological and mechanical evaluation of the small-diameter tissue-engineered blood vessels matrix

作　　者：谭菊[1] 曾文[1] 周静婷[1] 李刚[1] 张晓彦[1] 朱楚洪[1] 

机构地区：[1]第三军医大学基础部人体解剖学教研室,生物力学与组织工程重点实验室,重庆400038

出　　处：《局解手术学杂志》2014年第1期30-33,共4页Journal of Regional Anatomy and Operative Surgery

基　　金：国家自然科学基金(31200731);重庆市自然科学基金(cstc2011jjA0657)

摘　　要：目的研制一种具备正常血管的生理结构和良好的生物相容性,而且具有血管力学特性的小口径组织工程血管基质材料,并按医用输血材料的力学及生物学国家标准评价。方法以去细胞生物衍生材料作为基质,按照国家标准对其进行力学和生物学评价。结果血管的轴向、径向拉伸应力分别为23.14 N和36.79 N,均大于标准规定的7.5 N;拉伸率分别为95.19%和80.24%,大于标准规定的20%。血管的缝合强度为13.71 N,不小于0.5 N,血管符合力学规定。生物学检测主要用血管或其浸提液检测血管的pH值在对照去离子水pH(7.52±1.5)范围内、溶血率1.397 2%,小于5%、全血凝固时间比对照长50%、对细胞毒性为0级,皮内注射无刺激。结论血管基质材料具有良好的力学及生物学性能,它将在小口径血管替代治疗中起重要作用。Objective To develop a small-diameter tissue-engineered blood vessels which possesses normal blood vessels physiological structure, good biocompatibility, and mechanical properties. And it was evaluated by mechanical and biological of national standard of medi- cal transfusion material. Methods The bio-derived material were regarded as the ground substance, and it was evaluated by mechanical and biological of national standard after composite modification. Results The axial and radial tensile stress of the blood vessel was 23.14 N and 36.79 N respectively, and it was greater than the standard 7.5N. The tensile rate of the axial and radial was 95.19% and 80.24% respec- tively, which were higher than the standard value 20%. The suture strength of the blood vessel was 13.71 N, which was conform to the me- chanical requirement. Mainly used blood vessels or its extracts to detect the pH of the blood vessels is in the scope of control deionized water pH （7.5 ＋ 1.5 ） ; the hemolysis rate was 1. 3972% which was less than 5% ; the whole blood coagulation time was 50% longer than the con- trol level, and there was no stimulation after intradermal injection. Conclusion With bio-derived material as the ground substance and com- positely modified, this kind od blood vessels is conform to the mechanical and biological of national standard, and it has the potential of clini- cal application which could play an important role in the replacement therapy of small-diameter vascular xenografts.

关 键 词：小口径组织工程血管基质材料 力学性能 生物相容性 

分 类 号：Q819[生物学—生物工程] R322[医药卫生—人体解剖和组织胚胎学]