淫羊藿苷延长秀丽线虫寿命与机制  

Effect of icariin on lifespan of Caenorhabditis elegans

作　　者：吴俊珍 夏世金[2] 孙涛[1] 蔡外娇[3] 黄建华[3] 宁友[3] 吴金峰[3] 彭正羽[4] 

机构地区：[1]复旦大学附属华东医院老年医学科,上海市200040 [2]上海市老年医学研究所 [3]复旦大学附属华山医院中西结合研究所,上海市200040 [4]复旦大学生物医学研究院,上海市200032

出　　处：《老年医学与保健》2013年第6期349-352,364,共5页Geriatrics & Health Care

基　　金：国家自然科学基金（31171129）

摘　　要：目的探讨淫羊藿苷（Icariin，Ica）对秀丽线虫寿命的干预效果与机制，为筛选延缓衰老、延长寿命的药物提供实验依据。方法将秀丽线虫分为空白对照组、Ica5mg／L、10mg／L、15mg／L、20mg／L各剂量干预实验组，观察在Ica不同梯度剂量作用下秀丽线虫寿命的变化，以明确Ica作用的最佳剂量。接着设立秀丽线虫空白对照组和Ica（15mg／L）干预组，评价Ica对秀丽线虫平均寿命、生殖能力的干预效果；同时进行急性热应激和急性氧化应激实验，观察应激时线虫的平均生存时间，探讨Ica延长线虫寿命的机制。结果15mg／L的Ica是延长秀丽线虫寿命的最佳剂量。Ica组线虫的平均寿命明显长于空白对照组[（31．87±4．46）d vs．（20．13±2．81）d]（P〈0．01）；Ica组线虫生殖高峰期子代数目比空白对照组显著增加（151±21．13VS．122±17．09，P〈O．01）；急性热应激实验和急性氧化应激实验发现Ica组线虫平均生存时间均明显长于空白对照组[（9．53±1．34）hVS．（5．97±0．84）h，（8．34±1．17）hvs.（5．77±O．81）h]（P〈0．01）。结论淫羊藿苷能延长秀丽线虫的寿命，其机制可能与淫羊藿苷提高线虫的应激能力有关。Objective To evaluate the effect of Icariin （Ica） on lifespan of C. elegans in order to screen drugs for longevity. Methods （1） Dosage-effect test： C elegans, which were treated with Ica 0 mg/L, Ica 5 rag/L, 10 mg/L, 15 mg/L, 20 mg/L, respectively, were tested by lifespan assay to determine the best effective dosages of Ica. （2） Lifespan assay： C. elegans were divided into control group and Ica （15mg/L） treatment group. Lifespan assay was used to evaluate the life-extension effect of Ica on C elegans. （3） Fecundity test： C elegans fecundity in the Ica （15mg/L） treatment and control groups was assessed by evaluating the effect of Ica on the worms＇ reproduction. （4） Stress resistance test： the lifespan of C elegans in the Ica （15 mg/L） treatment and control groups were assessed by acute heat and oxidative stress treatment to explore the initial mechanisms involved in the effect of Ica on lifespan. Results 15 mg/L of Ica was the best dosage to made C elegans get the maximum lifespan. The average lifespan of C. elegans in the Ica-treated group was sig- nificantly longer than the control group [（31.87±4.46） d vs. （20.13±2.81） d ] （P〈0.01）. Compared with the control group, the C. elegans worms＇ reproduction of the Ica-treated group was significantly enforced （ 151 ± 21.13 vs. 122 ± 17.09 ） （P〈0.01）. Ica significantly extended the average survival time of C. elegans as compared with the control group under the acute heat and oxidative stress conditions [（9.53± 1.34） h vs. （5.97±0.84） h, （8.34± 1.17） h vs. （5.77±0.81） h] （P 〈 0.01 ）. Conclusion Icariin has a good effect on anti-aging and longevity of C elegans and the mechanism may involve in the improvement of heat and oxidative stress resistance ability of C elegans.

关 键 词：寿命 衰老 秀丽线虫 淫羊藿苷 热应激 氧化应激 生殖能力 

分 类 号：R339.8[医药卫生—人体生理学]