复方三金胶囊体内抗肿瘤作用研究  

Antitumor Effects of Fufang Sanjin Capsule on Human Breast Cancer In Vivo

作　　者：王宽宇[1] 覃迅云 李凤金[1] 武爽[1] 纪博硕 尤艳芳 刘弘涛[3] 牛雯颖[1] 肖洪彬[1] 

机构地区：[1]黑龙江中医药大学,黑龙江哈尔滨150040 [2]黑龙江德坤瑶医药研究院,黑龙江大庆163311 [3]黑龙江省药品审评认证中心,黑龙江哈尔滨150090

出　　处：《中医药学报》2013年第6期41-43,共3页Acta Chinese Medicine and Pharmacology

基　　金：黑龙江省博士后资助项目(2011ZX11102)

摘　　要：目的:研究复方三金胶囊的体内抗肿瘤作用。方法:32只Balb/c-n/n裸小鼠,皮下接种人乳腺癌细胞悬液后,随机分为4组即复方三金胶囊高、低剂量组、环磷酰胺阳性药对照组、模型对照组,每组8只;复方三金胶囊高、低剂量组给药量为4、2g/kg/d,环磷酰胺为0.03g/kg/d,模型对照组给以等体积蒸馏水,每天给药1次,持续给药14天。观察复方三金胶囊对荷瘤小鼠移植瘤质量、瘤体积、体质量和脾脏质量的影响。HE染色观察给药后瘤组织病理变化。结果:与模型组比较,复方三金胶囊高、低剂量组具有不同程度的抑制移植瘤增长的作用,抑瘤率为30.64%和11.26%;复方三金胶囊高、低剂量组小鼠的体质量、胸腺质量及指数降低,但无显著性差异,且均明显高于环磷酰胺组。HE染色结果表明,与模型组比较,复方三金胶囊高、低剂量组瘤组织中细胞碎凋亡细胞、坏死细胞明显增多。结论:复方三金胶囊具有一定的体内抗肿瘤作用。Objective： To study the antitumor effect of Fufang Sanjin capsule on human breast cancer in vivo. Method： After being injected MCF -7 cells, thirty - two nude mice were randomly divided into four groups i. e. model group, low dose of FFSJ capsule group, high dose of FFSJ group and positive control group （ n = 8 in each group, female）. model groups were treated by water with 0.2ml, experimental groups were treated with FFSJ at the dose of 4, 2g/kg/d and positive control group was treated with CTX at the dose of 0.03g/kg/d tbr 14 successive days respectively. Tumor inhibitive rate, tumor volume, body and spleen weight were observed. Result： Different doses of FFSJ capsule showed different levels of antitumor effects, while the weight of body and spleen almost did not change. The result of HE staining showed that the number of apoptotic and necrotic cells in tumor tissue increased after the treatment by FFSJ. Conclu- sion： FFSJ capsule has certain antitumor effects.

关 键 词：复方三金胶囊 荷瘤小鼠 乳腺癌 抑瘤率 HE染色 

分 类 号：R285.5[医药卫生—中药学]