牛四倍体胚胎的制备  

Production of bovine tetroploid embryos

作　　者：吕鑫[1,2] 杜卫华[2] 朱化彬[2] 马友记[1] 李发弟[1] 王宗礼[2] 

机构地区：[1]甘肃农业大学动物科学技术学院,甘肃兰州730070 [2]中国农业科学院北京畜牧兽医研究所,北京100193

出　　处：《甘肃农业大学学报》2013年第6期1-5,12,共6页Journal of Gansu Agricultural University

基　　金：国家奶牛产技术体系项目(CAR-37);国家科技支撑计划项目(2011BAD19B04)

摘　　要：为研究牛四倍体胚胎制备的条件及发育程度,以牛卵母细胞为试验材料进行体外受精(in vitro fertilization,IVF),受精后统计处于2-细胞期的胚胎数量,选取3个组合进行2-细胞期胚胎的融合,并统计融合率和融合后四倍体胚胎的存活率、卵裂率和囊胚率.结果表明:受精卵培养至14h时卵裂率最高,达到46.28%,因而确定为四倍体胚胎制备的开始时间.100V/mm,20μs,1次脉冲组的融合率(67.92±9.76)%、四倍体胚胎的存活率(93.71±5.31)%、卵裂率(70.37±7.07)%和囊胚率(19.44±3.62)%都显著高于其他2个组.因此,牛体外受精卵在mCR1aa培养液中培养14h后用100V/mm,20μs,1次脉冲进行四倍体的制备效果最佳.The aim of present study was to investigate best conditions for generating tetroploid embryos ac-cording to the cleavage rate and subsequent embryonic development during bovine invitro fertilization （IVF）.All the 2-cell embryos were counted and fused to generate tetroploid embryos after IVF.The fusion rate,survival rate, cleavage rate and blastocyst development of tetroploid embryos were evaluated.The results showed that the highest cleavage rate （46.28%）of zygotes appeared at 14 hours after in vitro culture.The fusion rate （67.92±9.76）%, survival rate （93.71± 5.31）%,cleavage rate （70.37±7.07）% and blastocyst rate （19.44±3.62）% of tetroploid embryos in the group with a single direct pulse of 100 V/mm for 20μs was significantly higher than other groups. Therefore,it was best to generate tatroploid embryos from bovine blastomeres that were cultured for 1 4 h and fused with a single direct pulse of 100 V/mm for 20μs.

关 键 词：牛四倍体胚胎 电融合 电压强度 脉冲数 

分 类 号：S814.8[农业科学—畜牧学]