葡萄品种亲缘关系的RAPD标记分析  

Genetic relationship among grape varieties based on RAPD analysis

作　　者：丁洁菲 毛娟[1] 赵举鹏[1] 陈佰鸿[1] 

机构地区：[1]甘肃农业大学农学院,甘肃兰州730070

出　　处：《甘肃农业大学学报》2013年第6期82-87,共6页Journal of Gansu Agricultural University

基　　金：甘肃省科技支撑计划(090NKCAO58)

摘　　要：利用RAPD分子标记技术对22个葡萄品种的亲缘关系进行了分析.研究表明:8个RAPD引物在22个葡萄品种中共扩增出162条带,其中多态性带为121条,多态率高达74%.采用UPGMA聚类分析法构建的系统树,在相似系数为0.425时,将22个葡萄品种分为6类.葡萄品种间的遗传多样性有明显差异.遗传差异小、亲缘关系近的品种很早就聚在一起,如‘黑大粒’和‘前项无核’;而有些品种,如‘藤稔’和‘高妻’,虽然同属巨峰系葡萄且均为同母本的杂交后代,聚类分析却分属于2大类,说明有性杂交的基因重组可表现出不同的基因型.Twenty-two genotypes of grapes （Vitis L.）were analyzed with RAPD.The results showed that eight RAPD primers could be amplified 162 bands in 22 grape varieties.There were 121 polymorphic bands,which occupied as high as 74%.When the similarity coefficient was 0.425,the phylogenetic tree constructed by UPGMA cluster analysis could divide the 22 grape varieties into 6 categories.The genetic polymorphism of grape was obvious.The cultivars which owned small genetic differences and close genetic relationship were clustered together early like ‘Exotic’and ‘Pre-entry Seedless’;some cultivars,such as‘Takatsuma’and ‘Fujimineri’,were hybrid parentage having the same female parent,at the same time, they belonged to ‘Kyoho’grapevine series.But they were clustered into two classes.It is suggested that on the condition of hybrid parentage,the crossed progeny could express disparate genotype.

关 键 词：葡萄 RAPD 亲缘关系 聚类分析 

分 类 号：S663.1[农业科学—果树学]