肝脏原位注射新生大鼠肝细胞构建的工程化肝组织对大鼠肝纤维化的影响  

Engineered hepatic tissue based on newborn rat liver cells improved liver fibrosis in rats by orthotopic injection

作　　者：周海洋[1] 郑爱民[1] 黄蓉蓉[1] 戴大江[1] 张海宏[1] 曹厚军[1] 

机构地区：[1]空军总医院普通外科,北京100142

出　　处：《空军医学杂志》2013年第3期149-151,166,共4页Medical Journal of Air Force

基　　金：国家863高技术研究发展计划资助项目(2009AA 043801)

摘　　要：目的观察原位注射新生大鼠肝细胞构建的工程化肝组织对实验性肝纤维化大鼠血清和肝组织学的影响,探讨其抗肝纤维化的作用。方法用四氯化碳制成实验性肝纤维化造模大鼠,肝脏原位多点注射工程化肝组织,3周后测定血清肝功能并行肝组织学检查。结果原位注射组大鼠肝功能较单纯假手术组大鼠有改善,且具有显著差异(P<0.05),HE染色提示纤维化明显改善。结论肝脏原位多点注射新生大鼠肝细胞的工程化肝组织能改善大鼠肝纤维化进程,有一定抗肝纤维化作用。serum and liver injection of hep Objective To observe the fibrosis index of experimenta atic constructs based on neon effecting on the content of liver function in 1 liver fibrogenesis rats, and investigate wether atal rat is provided with anti-liver fibrogenesis effect. Methods The rats were divided into two groups, and in group one, engineered hepatic grafts were implanted by orthotopically multi-spot injection. Liver cells isolated from from neonatal SD rats were mixed with liquid collagen prepared from SD rat tails.While group two received the same volume of saline solution.Liver function was evaluated by serum biological analysis at 10days. After 20days rats were killed, fibrosis index was examined by HE staining and Sirius red staining of liver tissue. Results Compared to controls, injection of hepatic constructs based on neonatal rat improve the liver function. Moreover, fibrosis index got more improvement in cell-matrix mixture than that in control group. Conclusion Multi-site injection of hepatic constructs based on neonatal rat liver cells in the liver can protect against experimental liver fibrosis in CC14-induced rats and the mechanisms will be studied further.

关 键 词：新生大鼠肝细胞 肝组织工程 肝纤维化 原位注射 

分 类 号：R657.3[医药卫生—外科学]