姜黄素对宫颈癌淋巴转移动物模型干预效应研究  

Effects of Curcumin on Lymphatic Metastasis in Human Uterine Cervical Cancer Animal Model

作　　者：汪茜[1] 李李[1] 汪宏波[2] 

机构地区：[1]南华大学附属第一医院妇产科,湖南衡阳421001 [2]华中科技大学附属协和医院妇产科,湖北武汉430022

出　　处：《湖南环境生物职业技术学院学报》2013年第4期17-20,共4页JOurnal of Hunan Environment Biological Polytechnic

摘　　要：目的研究姜黄素对IGF-1调控的宫颈癌淋巴转移动物模型的研究.方法建立宫颈癌淋巴转移鸡胚尿囊膜(CAM)模型,并观察姜黄素对IGF-1调控的淋巴管生成的作用.结果①在健康受精的SPF鸡胚的CAM上接种不同浓度的Hela细胞悬液1×103/L^1×108/L存活率差异没有统计学意义.1×107/L为最适接种浓度.②移植瘤组织切片HE染色课件癌细胞聚集成癌巢,与人宫颈癌形态相似.免疫组化染色,可见移植瘤内有微淋巴管形成③对照组、IGF-1组、Cur组和IGF-1+Cur组的LMVD依次为21.28±10.13、28.58±12.26、16.23±8.76和18.23±9.36条/4HP,LVA依次为2.91±1.16、3.42±1.25、2.00±1.51和2.72±1.21%,差异具有统计学意义(P<0.05).结论在鸡胚人宫颈癌移植瘤模型中可观察到姜黄素对IGF-1调控的宫颈癌淋巴管生成的抑制作用.Objective To study the effects of curcumin on the Lymphatic Metastasis in Human Uterine Cervical Cancer animal model. Methods： The CAM model of lymphatic metastasis in cervical cancer was established to study the effects of curcumin on IGF-1 related Lymphatic Metastasis of xenotransplanted tumor and the lymphatic microvessel density （LMVD）. Moreover, lymphatic vessel area （LVA） was measured and analyzed in this model. Results （~）We successfully established the chick chorioallantoic membrane （CAM） model of lymphagenesis in cervical cancer and found that 107/L was the optimal con- centrations of implantation of cancer cells in vitro. （2）The microlymphatics could be observed in intratumor in the CAM （Immune histochemistry staining）. （3）LMVD of the control, IGF-1, Cur and IGF-1 ＋ Cur groups were 21.28 ± 10. 13,28.58 ± 12.26,16.23 ± 8.76 and 18.23 ± 9.36/4HP respectively. Similarly, LVA were 2.91 ±1.16,3.42 ±1.25,2.00 ±1.51 and 2.72±1.21% in the control, IGF-1, Cur and IGF-1 ＋ Cur groups （p 〈0. 05 ）. Conclusion The expression of VEGF-C was up-regulated by IGF-1 in cervical cancer, which could be inhibited by addition of curcumin. The present research may imply in clinical application with Cur to inhibit the lymphagenesis in cervical cancer in future. 3figs. , 3 tabs. ,9refs.

关 键 词：姜黄素 宫颈癌 淋巴转移 鸡胚绒毛尿囊膜 移植瘤 IGF-1 VEGF-C 

分 类 号：R972.6[医药卫生—药品]