脱氢表雄酮对雄性小鼠睾丸组织发育的毒性作用  

Toxic Effect of Dehydroepiandrosterone on the Testis Tissue Development of Male Mice

作　　者：吕锦芳[1] 姜锦鹏[1] 宁康健[1] 应如海[1] 冯保明[1] 檀永强 

机构地区：[1]安徽科技学院动物科学学院,安徽凤阳233100

出　　处：《畜牧与饲料科学》2013年第12期27-30,共4页Animal Husbandry and Feed Science

基　　金：安徽省自然科学基金项目(11040606M88);安徽科技学院兽医学重点学科基金项目(AKXK20101-2)

摘　　要：昆明种雄性小鼠32只,随机分为4组。脱氢表雄酮(DHEA)日摄取量分别为0(对照组)、20、40和60 mg/kg,试验期52 d。各组分别在42日龄、72日龄处死小鼠,分离睾丸,制作石蜡切片,HE染色,显微观察、摄影。结果显示,与对照组比较,42日龄时中、高剂量组的曲精小管断面变形,各时期的生精细胞和成熟精子数量均减少;高剂量组曲精小管内生精细胞层次基本消失,出现较大腔隙,间质发生不同程度的变化。72日龄时中、高剂量组的曲精小管断面变形,基膜变薄伴有断裂和间质受损增加。中剂量组出现畸形精子,高剂量组曲精小管中央空白区域增大,缺乏成熟精子。以上结果表明,中、高剂量的DHEA对生长期小鼠的睾丸组织结构发育有明显的毒性作用。32 Kunming male mice were randomly divided into 4 groups,which were fed with DHEA of 0 mg/kg （control group）, 20 mg/kg（low-dose group）, 40 mg/kg （medium-dose group） and 60 mg/kg （high-dose group） every day. The experiment lasted for 52 days. The mice in every group were killed on the 42 day-old and 72 day-old respectively. The testis was picked to make into paraffin sections and observe in microscope after HE staining. The results showed that the cross-section of seminiferous tubules deformed on 42 day-old and the number of spermatogenic cells and mature spermatozoa in each period all reduced in medium-dose group and high-dose group, compared with control group. In high-dose group, the hierarchy of spermatogenic cell in seminiferous tubule basically disappeared, bigger lacuna appeared and interstitium changed to different extent. On 72 day-old, the cross-section of seminiferous tubules deformed, basement membrane became thin accompanied by fracture and damaged extent of interstitium increased in medium-dose group and high-dose group. Abnormal sperms were found in medium dose group. In high-dose group, central blank area in seminiferous tubule was enlarged and mature sperm lacked. The results suggested that medium-dose and high-dose of DHEA had obvious toxic effects on the structure development of testieular tissue in growing mice.

关 键 词：脱氢表雄酮 小鼠 睾丸 毒性作用 

分 类 号：Q577[生物学—生物化学] R927[医药卫生—药学]