马齿苋总黄酮对氧化应激损伤血管内皮细胞的影响  

Protective effect of portalaca total flavone on the injury of human vascular endothelial cells induced by hydrogen peroxide in vitro

作　　者：卢新华[1] 龚咏晴 曾中山 王桂霞[1] 韩瑛[1] 孙陶利[1] 郭皖北[1] 

机构地区：[1]湘南学院基础医学部,湖南郴州423000 [2]湘南学院化生系,药学2010级湖南郴州423000

出　　处：《湘南学院学报（医学版）》2013年第4期13-16,共4页Journal of Xiangnan University（Medical Sciences）

摘　　要：目的观察马齿苋总黄酮对过氧化氢诱导人血管内皮细胞氧化损伤的保护作用。方法采用体外培养人脐静脉内皮细胞(HUVEC),实验分为5组:正常对照组、过氧化氢损伤组、马齿苋总黄酮低、中、高剂量组。MTT法测定各组细胞活力,荧光分光光度计测量各组细胞线粒体膜电位水平。结果与正常对照组比较,过氧化氢损伤组血管内皮细胞活力、线粒体膜电位显著降低(P<0.01);与过氧化氢损伤组比较,马齿苋总黄酮干预组血管内皮细胞活力、线粒体膜Ratio值明显升高(P<0.05),且存在明显的剂量依赖关系。结论马齿苋总黄酮对氧化应激诱导血管内皮细胞损伤具有一定的保护作用,其机制可能与其抗氧化、维持线粒体膜电位有关。Objective To explore the protective effects of portalaca total flavone（FIT） on human vascular en- dothelial cell （HUVEC） injury induced by oxidative hydrogen in vitro. Methods Human vascular endothelial cells was cuhured in vitro and divided into 5 groups： control group, hydrogen peroxide injury group, PTF low, medium and high dose group. Cellular vitality of all groups was determined by MTF method, level of mitochondrial mem- brane potential measured by fluorescence spectmphotometer. Results Compared with control group, cellular vitality and mitochondrial membrane potential of hydrogen peroxide injury group decreased significantly （ P 〈 0.01 ）. Compared with hydrogen peroxide injury group, cellular vitality and mitochondrial membrane potential of portulaca flavonoids intervention group was significantly higher （ P 〈 0.05 ）, and with obviously dose - dependent relation- ship. Conclusion Portalaca total flavone（PTF）has certain protective effect on human vascular endothelial cell in- jury induced by oxidative hydrogen in vitro and its mechanism may be related to its antioxidant, maintain mito- ehondrial membrane potential.

关 键 词：马齿苋总黄酮 过氧化氢 人脐静脉内皮细胞 线粒体膜电位 

分 类 号：R285.5[医药卫生—中药学]