光动力疗法对人乳腺癌MCF-7细胞增殖凋亡的影响  

Effect of photodynamic therapy on the proliferation and apoptosis of human breast carcinoma cell line MCF-7

作　　者：王宏[1] 张洪梅[1] 阴慧娟[1] 顾立超 陈洪丽[1] 杨基春[1] 王磊[1] 李迎新[1] 

机构地区：[1]中国医学科学院北京协和医学院生物医学工程研究所,天津300192

出　　处：《国际生物医学工程杂志》2013年第6期321-323,I0007,共4页International Journal of Biomedical Engineering

基　　金：国家自然科学基金资助项目（31271434,81201819）;中央高校基本科研业务费专项资金资助项目;协和青年基金资助项目（3332013061）

摘　　要：目的 研究光动力疗法（PDT）对人乳腺癌MCF-7细胞增殖的影响和诱导凋亡的作用.方法 癌光啉（PSD-007）与细胞共同孵育2h后,以不同能量635 nm激光照射,通过噻唑蓝（MTT）比色法测定PDT后不同时间（0.5、1、3、6、12、24h）细胞的光密度（0D）值及存活率.DAPI染色观察PDT后不同时间细胞凋亡中细胞核形态学的改变.Annexin-V/PI双染法结合流式细胞术分析细胞凋亡率的变化.结果 PDT对MCF-7细胞的增殖有抑制作用,且随着PDT光照能量的提高而增强,PDT作用后细胞存活率随时间延长而逐渐降低.细胞形态学观察结果表明,MCF-7细胞呈典型的凋亡形态特征.Annexin-V/PI双染也证实PDT可以诱导细胞凋亡,且凋亡率随PDT作用后时间的延长而升高.结论 PDT能显著抑制MCF-7细胞增殖,诱导细胞凋亡,且其诱导肿瘤细胞的凋亡是一渐进性的过程,具有光照剂量和时间效应关系.Objective To observe the effects of photodynamic therapy （PDT） on proliferation and apoptosis of human breast carcinoma cell line MCF-7 in vitro.Methods MCF-7 cells were treated with PSD-007 for 2 h under the influence of low-level laser （635 nm） therapy at different doses.Then the optical density （OD） values and survival rates of MCF-7 cells were measured by MTT assay at different time （0.5,1,3,6,12,24 h） after PDT.The nucleus morphological changes of the MCF-7 cells stained by DAPI were observed under the reversal fluorescence microscope.The apoptosis was analyzed using flow cytometry through Annexin/PI double-labeled staining.Results PSD-007 mediated PDT inhibited the proliferation of MCF-7 cells,which depended on laser power and time after PDT.The morphological study proved that the multinucleate giant cells existed in the apoptotic cells in PSD-007 PDT.Flow cytometry of Annexin/PI double-labeled staining verified the results as well.Conclusion PDT inhibit the proliferation of MCF-7 cells in vitro by inducing apoptosis,and the results show an effect of time and dose dependence.

关 键 词：光动力疗法 细胞凋亡 乳腺癌 MCF-7细胞 癌光啉 

分 类 号：R454.2[医药卫生—治疗学] R737.9[医药卫生—临床医学]