IGF-1通过ERK-MNK信号通路诱导骨髓间充质干细胞VEGF表达的实验研究  

IGF-1 induces VEGF expression by ERK-MNK signaling pathway in bone marrow stromal cells

作　　者：刘柏含 高艳霞[2] 王占义[1] 姬朝光[2] 宫培刚 

机构地区：[1]长春市口腔医院颌面外科,吉林长春130051 [2]吉林大学基础医学院,吉林长春130021

出　　处：《中国实验诊断学》2014年第1期22-24,共3页Chinese Journal of Laboratory Diagnosis

基　　金：吉林省卫生厅项目基金支持;项目编号:2009z083

摘　　要：目的探讨ERK-MNK信号通路是否参与IGF-1对骨髓间充质干细胞VEGF表达的诱导。方法免疫印迹方法检测IGF诱导的骨髓间充质干细胞内HIF-1α、p-MNK等信号蛋白的表达变化和VEGF表达的变化,给予MNK抑制剂CGP 57380和HIF-1α抑制剂YC-1后再检测含外源性IGF-1的骨髓质间充质细胞中VEGF的表达水平。结果外源性IGF诱导后,HIF-1α、p-MNK等相关信号蛋白表达上调,分别阻断MNK和HIF后,VEGF的表达趋于正常。结论 IGF-1通过调节ERK-MNK信号通路诱导骨髓间充质干细胞BMSCS表达VEGF。Objective IGF-1 is one of the most abundant growth factors in bone marrow stromaI cells, which , as well as VEGF＇, plays an important role in promoting new bone formation or bone healing process. To investigate how to induce VEGF, exogenous IGF 1 was added to bone marrow stromal cells in vitro, then some signaling protein Inhibi tors was used to determine the role of signaling proteins related. Methods lay western blot, the expression of VEGF and signaling proteins-HIF-la, p-MEK, p-ERK, p-MNK and p-elF 4E was detected. The experiment was divided into four groups： （1） control group, （2） 50 ng/ml IGF-I,（3） CGP 57380- MNK inhabitor, and （4） 50 ng/ml IGF-1 and 5ng/ml HIF lainhabitor YC-1. Results Expression of these sinaling proteins and VEGF were upgraded after exoge- nous IGF 1, while increasing expression of VEGF is blocked by MNK inhabitor or HIF-linhabitor. Conclusion IGF- 1 regulated the expression of VEGF through ERK-MNK signal transduetion.

关 键 词：胰岛素样生长因子-1 血管内皮生长因子 ERK-MNK 骨髓间充质干细胞 骨组织工程 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]