Survivin维持小鼠胚胎干细胞多潜能性的研究  

Effects of Survivin on Maintenance of ES Cell Pluripotency

作　　者：张晶晶[1] 李晓晶[1] 李嘉欣[1] 冯若鹏[1] 

机构地区：[1]包头医学院生物化学与分子生物学教研室,内蒙古包头014040

出　　处：《动物医学进展》2014年第2期58-62,共5页Progress In Veterinary Medicine

基　　金：内蒙古自治区高等学校科学研究项目(NJZY12228);内蒙古自治区自然科学基金(2012MS1142)

摘　　要：为研究Survivin对维持胚胎干细胞(ES细胞)多潜能性的影响,用1μmol/L RA作为培养条件诱导ES细胞分化,通过Western blot与real-time PCR方法检测Survivin在ES细胞分化过程中的表达变化情况及对ES细胞多潜能相关标志表达的影响,同时通过Caspase活性检测的方法检测caspase-3在该过程中的激活状态。通过观察过表达Survivin后的ES细胞在RA诱导分化过程中的多潜能分子变化及caspase-3激活状态,表明RA可以诱导ES细胞中Survivin表达,并随时间变化而降低;同时多潜能相关分子表达下调,caspase-3激活。而过表达Survivin后RA诱导ES细胞分化过程中caspase-3激活与过表达空载组相比水平降低,同时伴随多潜能相关分子表达降低幅度减小。说明Survivin可以通过抑制RA诱导分化过程中caspase-3的激活,从而维持ES细胞多潜能相关分子表达。To explore the influence of Survivin on ES cell pluripotency maintenance, the ES cells were cul- tured in medium containing 1 /mol/L RA, Survivin and pluripotency maintenance molecules were detected by Western blot or real-time PCR in the process of ES cell differentiation. Meanwhile, the caspase-3 activi- ty was detected by caspase-3 detection Kit. The caspase-3 activation level and the pluripotency molecule expression were compared in the Survivin over-expression and empty expression ES cells cultured in RA containing differentiation medium. Results showed that the Survivin expression and pluripotency related molecules in ES cells can be down-regulated by RA and caspase-3 was activated in this process. But when Survivin was over-expressed in ES cells, the caspase-3 activation was delayed and pluripotency related mol- ecules decreased less. The conclusion is that Survivin can prevent the activation of caspase-3 in RA induced ES differentiation, to maintain the expression of pluripotency related molecules in ES cells.

关 键 词：胚胎干细胞 存活素 多潜能 半胱氨酸天冬氨酸蛋白酶3 

分 类 号：S814.8[农业科学—畜牧学]