毛蕊花糖苷改善D-半乳糖致亚急性衰老小鼠脑损伤的作用  

Improvement of acteoside on cerebral injury in subacute aging mice induced by D-galactose

作　　者：高莉[1] 彭晓明 霍仕霞 林娟 闫明 张富春[1] 

机构地区：[1]新疆大学生命科学与技术学院新疆生物资源基因工程重点实验室,新疆乌鲁木齐830046 [2]新疆维吾尔自治区维吾尔医药研究所新疆维吾尔医方剂学重点实验室,新疆乌鲁木齐830049

出　　处：《中草药》2014年第1期81-85,共5页Chinese Traditional and Herbal Drugs

基　　金：新疆维吾尔自治区科研机构创新发展专项资金(2012015);新疆维吾尔自治区公益性科研院所基金(KY2012114)

摘　　要：目的研究毛蕊花糖苷对D-半乳糖致亚急性衰老小鼠脑损伤的改善作用。方法建立D-半乳糖致亚急性衰老小鼠模型,以其学习、记忆成绩,免疫器官指数,脑中胆碱乙酰转移酶(ChAT)和乙酰胆碱酯酶(AchE)活性,小鼠海马CA1区细胞形态为指标,对毛蕊花糖苷改善D-半乳糖致亚急性衰老小鼠脑损伤的作用进行研究。结果与模型组比较,毛蕊花糖苷可恢复D-半乳糖致亚急性衰老小鼠的学习记忆损伤;拮抗脑组织和免疫器官萎缩;提高脑中ChAT活性,降低AChE活性;改善脑组织海马CA1区椎体细胞的数量和排列。结论毛蕊花糖苷能明显改善D-半乳糖致亚急性衰老小鼠的学习记忆能力障碍,可以调节脑组织ChAT和AchE活性,保护脑组织海马CA1区神经元细胞,提高脑组织和免疫器官指数,改善模型小鼠的脑损伤,提示毛蕊花糖苷的作用可能与其增强中枢胆碱能功能和保护神经元细胞有关。Objective To investigate the effect of acteoside on cerebral injury in subacute aging mice induced by D-galactose. Methods The aging model of mice was established by sc administration of D-galactose, taking learning, memory, index of immune organs, activities of choline acetyltransferase （CHAT） and acetylcholinesterase （AchE） in mice brain, and cell morphology of hippocampal CA1 region as indicators to study the effect. Results Compared with the model group, acteoside could recover the learning and memory capacity, resist immune organs atrophy, enhance the activity of CHAT, inhibit the activity of AChE, and improve the number and arrangement of cerebral pyramidal cells in hippocampal CA1 region. Conclusion Acteoside has remarkably reduced the crerebral injury of mice induced by D-galactose, and could increase the activities of ChAT and AchE in mice brain, protect the brain cells in hippocampal CA1 region, improve immune organs index, and enhance the learning and memory capacity. Acteoside has the protective effects on cerebral injury induced by D-galactose, and the mechanism may be related to the improvement of the central cholinergic system and the protection of nerve cells.

关 键 词：毛蕊花糖苷 D-半乳糖 学习记忆能力 胆碱乙酰转移酶 乙酰胆碱酯酶 

分 类 号：R285.5[医药卫生—中药学]