黄芩及其同属近缘种的DNA条形码鉴定研究  

Authentication of DNA barcoding of Scutellaria baicalensis and its related species

作　　者：夏至[1] 冯翠元 高致明[1] 李贺敏[1] 张红瑞[1] 

机构地区：[1]河南农业大学农学院中药材工程技术研究中心,河南郑州450002

出　　处：《中草药》2014年第1期107-112,共6页Chinese Traditional and Herbal Drugs

基　　金：科技部国家星火计划项目(2012GA750004);国家自然科学基金面上项目(31070171;31270236)

摘　　要：目的利用DNA条形码鉴定技术，快速、准确地鉴别黄芩及其同属近缘种（易混品）。方法提取黄芩及其同属近缘种的DNA，对ITS和psbA．trnH序列扩增和测序，计算物种种内种间Kimura2-parameter（K2P）遗传距离。采用相似性搜索法、最近距离法、构建Neighbor-joining（NJ）系统聚类树等方法进行鉴定分析。结果黄芩及其同属近缘种种间的ITS和psbA-trnH’序列的遗传距离为0．06797～0．08880和0．05061～0．05737，明显大于黄芩种内的遗传距离0～0．00640和0～0．00311。ITS和psbA．trnH分子系统树显示，黄芩8个居群个体均聚为一单系分支，支持率均为100％，与其5个同属近缘种很好地区分开。3种鉴定方法表明，ITS和psbA．trnH序列适合鉴别黄芩及其同属近缘种。结论ITS和psbA．trnH序列可以有效准确鉴别中药材黄芩及其近缘种。Objective To fastly and acurrately authenticate Scutellaria baicalensis and its closely related species using DNA barcoding technique. Methods Total genomic DNA was isolated from S. baicalensis and its related species. Nuclear DNA ITS and chloroplast gene psbA-trnH sequences were amplified and sequenced. The Kimura 2-parameter （K2P） distances were calculated. Identification analyses were performed using BLAST1, nearest distance, and Neighbor-joining （NJ） methods. Results The genetic distances oflTS and psbA-trnH between S. baicalensis and its closely related species were （0.067 97- 0.088 80） and （0.050 61-0.057 37）, which were obviously higher than those in the intra-species ofS. baicalensis （0-0.006 40） and （0-0.003 11）. The NJ tree of ITS and psbA-trnH indicated that the eight geographical populations ofS. baicalensis formed a monophyletic clade [bootstrap （BS） = 100%]. All the three methods showed that ITS and psbA-trnH could discriminate S. baicalensis from its closely related species correctly. Conclusion ITS and psbA-trnH are two efficient barcodes for the authentication of S. baicalensis and its related species.

关 键 词：DNA条形码 ITS PSBA-TRNH 黄芩 同属近缘种 鉴定. 

分 类 号：R282.12[医药卫生—中药学]