HPLC法同时测定维血宁中3种化学成分的含量  

Simultaneous Determination of Three Kinds Chemical Contents in Weixuening by HPLC

作　　者：曹瑞[1] 谢锦艳[1] 柴江[1] 何瑞[1] 程茜菲[1] 宋小妹[1] 

机构地区：[1]陕西中医学院,陕西咸阳712046

出　　处：《中医药导报》2014年第1期84-85,88,共3页Guiding Journal of Traditional Chinese Medicine and Pharmacy

基　　金：陕西省重大科技创新项目(2009ZKC05-05)

摘　　要：目的:建立同时测定维血宁中虎杖苷、大黄素-8-O-β-D葡萄糖苷及大黄素含量的方法。方法:应用HPLC法测定,色谱柱:Kromasil C18Column(4.6 mm×250 mm,5μm);流动相:乙腈-0.1%磷酸溶液,梯度洗脱;流速:1.0 mL·min-1;柱温:30℃;检测波长:288 nm。结果:虎杖苷、大黄素-8-O-β-D葡萄糖苷和大黄素的线性范围分别为0.09-0.72μg(r=0.9984)、0.4825-3.86μg(r=1.0000)、0.2675-2.14μg(r=1.0000);平均加样回收率分别为98.9%(RSD%=2.8%)、99.6%(RSD%=0.16%)、98.1%(RSD%=0.44%)。结论:该方法同时测定维血宁中的虎杖苷、大黄素-8-O-β-D葡萄糖苷和大黄素的含量,简便易行、准确、重复性好,可为维血宁的质量控制研究提供一种可靠的参考方法。Objective： To establish a high performance liquid chromatographic method for simultaneous determination of the contents of Polydatin, Emodin-8-O-β-D-glycosidase and Emodin in Weixuening. Methods： HPLC was applied with the chro- matographic condition as follows; the chromatographic column was Kromasil C18 Column （4.6 mm×250 mm, 5 μm）; acetonitrile-0.1% H3PO4 as the mobile phrase with gradient elutian; flow rate was 1.0 mL.min-1; the detection wave length was 288 nm; temperature was 30 ℃. Results： Linearity relationships were 0.09-0.72 μg （r=0.9984）, 0.4825-3.86 μg （r=1.0000） and 0.2675- 2.14 μg （r=1.0000）, respectively of Polydatin, Emodin-8-O-β-D-glycosidase and Emodin, as well as the average recoveries of 98.9% （RSD%=2.8%）,99.6% （RSD%=0.16%） and 98.1% （RSD%=0.44%）. Conclusion： The results showed that the method is simple, accurate and repeatable and that it is suitable for simultaneous determining the contents of Polydatin, Emodin-8-O-β- D-glycosidase and Emodin in Weixuening.

关 键 词：HPLC 维血宁 大黄素-8-O-β-D葡萄糖苷 大黄素 虎杖苷 

分 类 号：R284.1[医药卫生—中药学]