核桃内生菌与病原真菌的体外互作研究  

A Study on the in vitro Interaction of Endophyte with Pathogenic Fungi in Walnut

作　　者：傅本重[1] 周攀[1] 王立华[1] 李国元[1] 邹礼平[1] 吴瑶[1] 

机构地区：[1]湖北工程学院生命科学技术学院/特色果蔬质量安全控制湖北省重点实验室,湖北孝感432000

出　　处：《江西农业大学学报》2013年第6期1173-1178,共6页Acta Agriculturae Universitatis Jiangxiensis

基　　金：国家自然科学基金项目(31200488);云南省应用基础研究计划项目(2011FB066);特色果蔬质量安全控制湖北省重点实验室开放基金项目(2013K02)

摘　　要：为挖掘核桃内生菌的生物防治潜能。对核桃的内生菌和病原菌进行了分离,并通过平板对峙和发酵液抑菌试验,对其体外互作进行了研究。结果发现3株核桃内生细菌XHE7、XHE8和XHE12对2种病原真菌HK2(Colletotrichium sp.)和HD1(Alternaria sp.)有拮抗作用,抑制率高达82%。其发酵液对HK2的抑制作用高达100%。对其中2株拮抗作用明显的细菌进行了生理生化和16S rDNA分子鉴定,初步确定XHE8和XHE12皆为芽孢杆菌(Bacillus sp.)。In order to exploring the biocontrol talent of walnut endophytic bacteria, several endophytes and tWO pathogens in walnut were isolated, and the in vitro interactions between endophyte and pathogens were studied by using plate-confrontation and zymotic fluid inhibition assay.The results showed that three endophyt- ic strains XHE7 ,XHE8 and XHEI2 represented significant antagonism to both HK2（ CoUetotrichium sp. ） and HD1 （Alternaria sp. ）, and the highest inhibition rate reached 82.01%.Their zymotic fluid could suppress HK2 growth rate as high as 100%. Combined physiological and biochemical characters with 16S rDNA sequencing results,both strains XHE8 and XHE12 were identified as Bacillus sp..

关 键 词：核桃 内生菌 体外互作 拮抗作用 16S RDNA 芽孢杆菌 

分 类 号：S763.11[农业科学—森林保护学]