犬瘟热病毒的蚀斑克隆试验  

Canine distemper virus strain colned with plaque forming test

作　　者：何洪彬[1] 夏咸柱[1] 黄耕[1] 范泉水[1] 邱薇[1] 余春[1] 乔军[1] 徐黎[1] 王雷[1] 

机构地区：[1]解放军军需大学兽医研究所,吉林长春130062

出　　处：《黑龙江畜牧兽医》2000年第9期3-4,共2页Heilongjiang Animal Science And veterinary Medicine

基　　金：军队"八五"医药卫生重点基金课题! (91- 0 2 0 5 )

摘　　要：对血清、胰酶以及染色时间等可能影响犬瘟热病毒蚀斑形成的条件进行了摸索。结果发现 ,对必须同步接毒的CDV小熊猫 (LP)株来讲 ,其形成蚀斑的最佳条件是 :同步接毒 10h后 ,加入血清含量为 2 %的无酚红的DMEM营养琼脂 ,第 5d时 ,加入 1/ 5体积的胰酶含量为 1/ 80 0 0的含中性红的DMEM营养琼脂染色 ,于第 6d时 ,便可形成蚀斑。挑选不同大小的蚀斑 ,克隆 3次后 ,获得了蚀斑直径为 0 .4～ 0 .6 ,0 .6～ 0 .8及 0 .7～ 0 .The in fluence factors on plaque forming test of caninedistemper virus(CDV) LP strain,such as the concentration of bovineserum,pancriatic and different staining time,were determined.The Verocells infected with CDV LP strain were cultured in Eagle's medumcontaining 2% newborn bovine serum and 1% agarose gel.After 5 days,the staining agarose gel were added and the plaque in monolayer Verocells with LP strain were formed until the sixth day.The plaque(0.7 to 0.9,0.6 to 0.8 and 0.4 to 0.6 mm)virus es were isol ated by threesequential plaque selections.

关 键 词：犬瘟热病毒 克隆 蚀斑试验 

分 类 号：S852.655[农业科学—基础兽医学]