Zn^(2+)对福寿螺肝脏抗氧化酶活性和脂质过氧化的影响  

Effects of Zn^(2+) on Antioxidant Enzymes Activities and Lipid Peroxidation of Hepatopancreatic in Pomaceacanaliculata

作　　者：柯佳颖[1] 王泽贤[1] 陈细香[1] 陈朝阳[1] 

机构地区：[1]泉州师范学院化学与生命科学学院,福建泉州362000

出　　处：《泉州师范学院学报》2013年第6期19-23,共5页Journal of Quanzhou Normal University

基　　金：泉州市第三批技术研究与开发项目(2010N28)

摘　　要：以福寿螺为研究对象,测定其在Zn2+浓度分别为0、1、2、5、10mg/L时,于不同暴露时间(12、24、48、96h)内,肝脏超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和丙二醛(MDA)活性的变化.结果表明:低浓度(1、2mg/L)的Zn2+处理组在24h内对福寿螺肝脏SOD活性起到诱导作用;随着时间的延长活性逐渐减弱,在96h时下降至最低值,极显著低于对照组水平;而中、高浓度(5、10mg/L)处理组酶活性均低于对照组.在各浓度组的胁迫下,CAT活性在12h时升高到最大值,之后随着时间的延长均表现为下降趋势.MDA含量在整个试验期间均处于诱导状态,并且与重金属浓度成正比,在10mg/L 96h时达到最大值.Zn2+会对福寿螺产生明显的氧化胁迫,SOD、CAT和MDA指标能有效评价Zn2+对福寿螺的氧化损伤.Abstract：Using [omacea canaliculata as the activities of superoxide dismutase an experimental animal, the effects of （SOD）, catalase （CAT） and the heavy metals Zn2＋ on content change malondialdehyde（MDA） in hepatopancreas were studied when treated for different times（12,24,48,96 h）and by different concentrations（0,1,2,5,10 rag/L） respectively. The results showed that the low concentration of Zn2＋ （1,2 mg/L） treatment group plays a role of inducing effect on SOD activity of hepatopancreatic in pomacea canaliculata within 24 h. As the extension of time,activity drops to the lowest in 96 h,significantly lower than control group, and the enzyme activity, medium and high con- centrations of treatment group were lower than the control group. Concentration in each group under the stress,the CAT activity increases at 12 h to the maximum,then over time are characterized by a downward trend. MDA content during the entire test in induced condition, and is proportional to the concentration of heavy metals,and reached the maximum in the concentration 10 mg/L of 96 h. Zn2＋ will have obvious oxidative stress on pomacea canaliculata, the results of various indicators Effective evaluationof Zn2＋ oxidative damage to pomacea canaliculata.

关 键 词：锌离子 福寿螺 超氧化物歧化酶 过氧化氢酶 丙二醛 

分 类 号：Q956[生物学—动物学]