超短波治疗对鼠断尾再植后鼠尾内皮素的影响  

Effects of ultra-short wave therapy on endothlin of anastomotic site after rat tail replantation

作　　者：谭龙[1] 高文山[2] 席阿丽[3] 杨信才[1] 瓮沛杉[4] 

机构地区：[1]河北大学附属医院康复医学科,河北保定071000 [2]河北大学附属医院骨科,河北保定071000 [3]兰州军区边防军医训练大队药化教研室,新疆呼图壁831200 [4]河北大学医学部,河北保定071000

出　　处：《医学研究与教育》2013年第6期5-12,共8页Medical Research and Education

基　　金：河北省2009年医学科学研究重点课题计划项目(20090573)

摘　　要：目的探讨超短波（ultra—shortwave，USW）治疗对鼠断尾再植后血管危象的影响及作用机制。方法动物实验从2009年10月至2010年6月实施。取3个月龄雌性成年sD大鼠80只，体质量232．8-289．6g，完全随机化分为5组。各组保留尾侧静脉、尾骨后断离尾，空白组结扎尾动脉；对照组、罂粟碱组、USW高剂量组、USW低剂量组均吻合尾动脉，建立断尾再植模型。术后对照组正常饲养管理，不给任何治疗措施。罂粟碱组术后立即腹腔内注射稀释的盐酸罂粟碱注射液。USW高剂量组、USW低剂量组术后20min立即给予局部吻合口处USW治疗，此后每天1次，至术后5d。USW高剂量组USW剂量：3档，50mA，20min。USW低剂量组剂量：2档，28mA，20min。术后观察鼠尾成活情况至术后10d。手术前后测鼠尾吻合口近远侧皮温差并计算术后与术前的变化值。术前从内眦及术后第8小时从尾尖各采血1次，测血浆内皮素（endothelin，ET）浓度并比较。结果鼠尾成活率：所有鼠分2组，断尾组（空白组）0％（0／14），断尾再植组（对照组-usw低剂量组）44％（29／66），组问差异有统计学意义（P〈0．05）。5组鼠尾成活率分别为0％（0／14）、36．4％（8／22）、57．1％（8／14）、22．2％（4／18）、75．0％（9／12），总体比较差异有统计学意义（P〈0．05）。断尾再植的各组（对照组、罂粟碱组、USW高剂量组、USW低剂量组）总体比较差异有统计学意义（P〈0．05）。断尾再植各组两两比较：USW低剂量组〉对照组，USW低剂量组〉USW高剂量组，罂粟碱组〉USW高剂量组，差异均有统计学意义（P〈0．05）；余2组间差异无统计学意义（P〉0．05）。术前鼠尾皮温差两两比较差异均无统计学意义（P〉0．05）。术后第8小时、5天、6天及7天鼠尾皮温差变化值各组差异均有统计学意义（P〈0．05）。两两比较：其中术后第8Objective To explore the effects and mechanism of ultra-short wave（USW） for prevention and treatment of vascular crisis after rat tail replantation. Methods The animal experiments were carried out from October 2009 to June 2010. Eighty 3-month old female Sprague Dawley rats （weighing 232.8-289.6 g） were randomly divided into five groups. In each group, based on the caudal vein and the coccyx was retained, the tail was cut off. The tail artery was ligation in Blank group. The tail artery were anastomosed in Control group, Papaverine group, USW high dose group and USW low dose group group to establish the tail replantation model. After surgery, the rats of the Papaverine group were immediately given papaverine hydrochloride injection. The rats of the USW high dose group and the USW low dose group were immediately given the local USW treatment at anastomotic site within postoperative twenty minutes, once a day thereafter, for 5 days at the dosage of three tranche and 50 mA for 20 min（high dose group） and two tranche and 28 mA for 20 min（low dose group）. The survival of the rat tails was observed after the tail replantation for 10 days. The difference between proximal and distal anastomosis site tail skin temperature were measured before and after the replantation; the change between postoperative and preoperative temperature difference was calculated. From the inner canthus before the replantation and from the collected blood plasma specimens and measured contents tip of the tail at postoperative 8th hour, rats were of endothelin. Results The survival rates of the rat tails were 0%（0/14） and 44% （29/66）in the tails cutting off group （Blank group） and the tail replantation group （Control group, Papaverine group, USW high dose group, USW low dose group） respectively, showing significant difference among two groups （5（2=9.649, P=-0.002）. The survival rates of the rat tails were 0%（0/14）, 36.4%（8/22）, 57.1%（8/14）, 22.2%（4/18）, 75.0%（9/12） in groups Blank, Control

关 键 词：断尾再植 血管危象 超短波 血管内皮细胞 内皮素 皮温差 

分 类 号：R68[医药卫生—骨科学]