抗稻瘟病菌活性木荷皂甙类似物的分离条件及其分离  

Separation of Saponin Analogue from Schima Superba with Activity Resisting Magnaporthe Oryzae

作　　者：彭玉萌 霍光华[1] 韩启灿[1] 詹五根[1] 叶亚健[1] 王纯荣[1] 

机构地区：[1]江西农业大学,南昌市生物资源保护和利用重点实验室,南昌330045

出　　处：《分析化学》2014年第1期59-64,共6页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金(Nos.31060250;21266010);江西省自然科学基金(No.20132BAB204028)资助~~

摘　　要：新型木荷各皂甙极性相似,结构相近,给分离带来了极大困难,制约了抗稻瘟病菌活性木荷皂甙先导物的确立。运用单因素(色谱柱填料、流速、流动相配比)探索其色谱行为,综合塔板理论、速率理论及线性放大原理推导,以及菌丝生长速率法测定活性,确定了木荷皂甙的色谱分析条件为:Hypersil BDS C18色谱柱,流速0.8 mL/min,特定的梯度洗脱程序,获得了较为满意的色谱分析图谱,推导出木荷皂甙的速率理论半经验公式为H=!23.5195×10!3+0.1155×10!1/u+14.9675×10!3u,应用到半制备HPLC,成功分离出7个木荷皂甙单体,其中2种具有强抗稻瘟病菌活性,其EC50/EC90分别为5.9123/21.6912,5.2064/16.9781。Due to the similar polarity and structure, it brought great difficulties for the separation of new Schima superba saponins, which restricts the establishment of active Schima superba saponin lead compounds that can resist magnaporthe oryzae. The chromatographic analysis conditions of Schima superba saponins were examined by exploring chromatographic behaviors with the help of single factor （packing material, flow rate, ratio of mobile phase） and determining activity through the application of composite plate theory, rate theory, linear amplification principle and mycelium growth rate method. The results were as followed： Hypersil BDS C18 chromatographic column, the flow rate was 0.8ml/min. Through a specific gradient elution procedure, we obtained a relatively satisfactory chromatographic analysis chart and deducted a velocity theory semi-empirical formula for Sc hima superba saponins, H = -23.5195 × 10 -3 ＋ 0.1155 × 10 -1 /u ＋ 14. 9675 ×10 -3 u, then applied it to semi-preparative HPLC, and by which seven Schima superba saponin monomers was separated successfully. The result shows that there are two kind of Schima superba saponin monomers with strong activity of resisting magnaporthe oryzae, and their ECS0/EC90 are 5. 9123/21. 6912, 5. 2064/16. 9781 respectively.

关 键 词：木荷皂甙 色谱行为 分离 抗稻瘟病菌活性 

分 类 号：S435.111.41[农业科学—农业昆虫与害虫防治]