金边阔叶麦冬(Liriope platyphylla Wang et Tangvar.variegata Hort.)的组织培养  

The tissue culture of Liriope platyphylla Wang et Tang var. variegata Hort.

作　　者：莫肖蓉[1] 朱诚[1] 任晓米[1] 唐宇力 钱萍[3] 

机构地区：[1]浙江大学生命科学院,杭州310029 [2]杭州市植物园,杭州310013 [3]杭州市园文局灵隐管理处,杭州310007

出　　处：《植物资源与环境学报》2000年第4期27-29,共3页Journal of Plant Resources and Environment

基　　金：杭州市园林文物管理局科技基金资助项目

摘　　要：将金边阔叶麦冬 (LiriopeplatyphyllaWangetTangvar.variegataHort.)不同部位外植体块 ,接种于附加不同激素配比的MS培养基上 ,实验结果表明 ,取自根状茎芽点 ,培养基为MS +BA 1.5mg/L +NAA 0 .5mg/L的不定芽诱导率最高 ( 10 0 % ) ,长势较旺 ,不定芽和愈伤组织均较多 ,但极易造成性状分离。用MS +BA 3.0mg/L +NAA 0 .5mg/L液体培养可以直接“芽生芽” ,避免了脱分化过程 ,从而稳定保持嵌合性。不定芽每月可转接 1次 ,适宜的生根培养基为 1/2MS大量 +IBA 0 .2 5mg/L。试管苗在蛭石中炼苗后即可移栽。The segments from various part of \%Liriope platyphylla \%Wang et Tang var. \%variegata \%Hort. were cultured on MS medium supplemented with variable ratio of hormones. The results showed that the highest induction frequency (100%) of adventitious buds was obtained by culturing the buds of rootstocks on MS medium supplemented with BA 1.5 mg/L+NAA 0.5 mg/L, but the mosaic of the plant can't be kept by this way. In order to solve this problem, the liquid culture with MS medium supplemented with BA 3.0 mg/L+NAA 0.5 mg/L can avoid dedifferentiation and keep the mosaic of the proliferated buds. The adventitious buds could be transplanted once a month, while MS medium supplemented with IBA 0.25 mg/L is suitable for rooting. After a period of training, the rooted plants could be planted on soil.

关 键 词：金边阔叶麦冬 组织培养 根状茎芽点 嵌合性 

分 类 号：S682.360.3[农业科学—观赏园艺] Q813.12[农业科学—园艺学]