CD_8^+CD_(28)^-调节性T细胞在上呼吸道感染后长期咳嗽发病机制的作用  

Role of CD_8^+CD_(28)^- regulatory T cells in patients undergoing chronic cough secondary to upper respiratory tract infection

作　　者：陈济明[1] 李一禄[1] 黄平[1] 周燕宁[1] 杜秀芳[1] 聂莉[1] 

机构地区：[1]广东医学院附属南山医院呼吸内科,广东深圳518052

出　　处：《临床肺科杂志》2014年第2期239-242,共4页Journal of Clinical Pulmonary Medicine

摘　　要：目的探索CD+8CD-28调节性T细胞在上呼吸道感染(URI)后长期咳嗽发病中的作用。方法将62例长期咳嗽患者分为轻中重3组,分别检测肺功能、外周血及痰液中嗜酸粒细胞(EOS)比例和CD+8CD-28T细胞含量及其细胞因子表达;同期选取22例健康体检者作为对照组。结果长期咳嗽患者肺功能呈不同程度受损,以重度咳嗽者明显。长期咳嗽患者的外周血及痰液中EOS比例、CD+8CD-28T细胞含量均显著高于健康对照组;其中重度咳嗽组EOS比例、CD+8CD-28T细胞含量显著高于轻、中度咳嗽组[EOS比例:外周血(4.86±2.79)%比(3.81±2.16)%、(4.32±2.27)%,痰液(19.38±7.89)%比(9.29±4.92)%、(13.21±5.86)%;CD+8CD-28T细胞含量:外周血(21.92±5.91)%比(12.98±4.74)%、(16.27±5.01)%,痰液(9.02±3.26)%比(3.84±1.06)%、(5.92±2.17)%,均P<0.05]。长期咳嗽患者CD+8CD-28T细胞上清液中细胞因子白细胞介素-10(IL-10)、转化生长因子-β1(TGF-β1)、叉头翼状螺旋转录因子(Foxp3)的mRNA表达均明显高于健康对照组,且随培养时间延长呈逐渐升高趋势,其中仍以重度咳嗽组最高。肺功能、EOS、CD+8CD-28T细胞及其细胞因子间均呈显著相关性。结论继发于URI的长期咳嗽患者存在气道高反应性,CD+8CD-28T细胞及其细胞因子可能参与该过程。Objective To explore the role of CD8^＋ CD28^- regulatory T cells in patients with chronic cough secondary to upper respiratory tract infection （URI）. Methods 62 subjects were divided into the mild, moderate and severe groups, respectively, and another 22 heahhy subjects were enrolled as the control group. Their lung function, induced sputum eosinophils （EOS） and blood CDs CD~ T cell levels were compared among the 4 groups. Results Their pulmonary function was defected for all the cough patients and the severe group were worse than the rest three groups （P 〈0. 05）. The levels of EOS and CDs CD~ T cells, and their cytokines including IL-10, TGF- 131 and Foxp3 were significantly higher in the patients group than in the control group, which was the highest in the severe group （P 〈0.05）. The levels of IL-10, TGF-131 and Foxp3 mRNA was the highest in the severe group, and it increased with culture time. Correlation analysis showed that CDs CD~ T cells and their cytokines were correlated with both pulmonary function and EOS. Conclusion Patients with chronic cough secondary to upper respiratory tract infection suffer from airway hyperresponsiveness （ AHR）, and CD8^＋ CD28^- regulatory T cells play a vital part in the process in AHR for these cough patients.

关 键 词：CD+8CD-28调节性T细胞 慢性咳嗽 气道高反应 哮喘 

分 类 号：R56[医药卫生—呼吸系统]