CIMMYT小麦Pavon 76和PBW 343叶锈成株抗性QTL分析  

QTL Mapping of Adult-plant Resistance Genes to Leaf Rust in CIMMYT Wheat Pavon 76 and PBW 343

作　　者：郑嫚嫚[1] 王翠芬[1] 李欢[1] 李在峰[1] 刘大群[1] 

机构地区：[1]河北农业大学植物保护学院,河北省农作物病虫害生物防治工程技术研究中心,河北保定071001

出　　处：《河南农业科学》2014年第1期74-78,共5页Journal of Henan Agricultural Sciences

基　　金：国家自然科学基金项目(30971772)

摘　　要：CIMMYT(墨西哥国际玉米小麦改良中心)小麦多数携带成株慢锈基因,Pavon 76和PBW343在田间对我国叶锈菌种表现为抗病,其可能携带有多个成株慢锈QTL位点。为了检测和定位Pavon 76和PBW 343中的成株慢锈QTL位点,以Pavon 76和PBW 343杂交、多次自交获得的包含有178个家系的重组自交系(RIL)群体为材料,将其种植在河北农业大学试验田和CIMMYT的Obregon小麦试验田,分别接种不同的小麦叶锈菌混合小种进行田间抗叶锈鉴定,获得群体的表现型数据,同时利用480个在亲本间有多态性的DArT(多样性序列芯片技术)标记检测178个家系,获得群体的基因型数据。结合表现型数据和基因型数据,利用Map Manager QTXb20和QTL IciMapping软件,进行复合区间作图法分析,在2个环境共检测到4个QTL位点。其中位于1BL染色体上来源于亲本Pavon 76的QTL位点解释5.5%的表型变异,另外3个QTL位点都来源于亲本PBW 343,其中的2个QTL位点位于1AL和2DL染色体上且只在墨西哥试验点检测到,分别解释7.0%和4.3%的表型变异,另外1个QTL位点只在保定试验点检测到,位于3AL染色体上,解释5.7%的表型变异。位于1A和3A染色体上的QTL可能为新的成株抗叶锈QTL,其丰富了现有的小麦成株慢锈基因库,与其紧密连锁的分子标记可以用于标记辅助育种、培育持久抗病品种。Most of CIMMTY wheat lines carry adult-plant slow rusting genes .Pavon 76 and PBW 343 show high resistance to Chinese Puccinia triticina pathotypes in the field and may carry several slow rusting genes .A total of 178 recombinant inbred lines （RILs）derived from the cross Pavon 76 and PBW 343 were planted in Agricultural University of Hebei and Obregon ,CIMMYT to score disease severity for leaf rust with mixed Puccinia triticina pathotypes of China and Mexico ,respectively .A total of 480 DarT markers with polymorphism between parents were used to test 178 RILs of the population for making genetic map .Phenotype data and genotype data were used to map slow rusting QTLs in the population by softwares Map Manager QTXb20 and QTL IciMapping with composite interval mapping strategy .The results showed that 4 adult-plant resistance QTLs were identified in two environments .One QTL located on 1BL deriving from Pavon 76 explained 5 .5% of the phenotypic variance .Two QTLs on 1AL and 2DL deriving from PBW 343 were detected in Mexico and explained 7 .0% and 4 .3% of the phenotypic variance ,re-spectively .The last QTL on 3AL deriving from PBW 343 was detected in Baoding and explained 5 .7% of phenotypic variance .The QTLs on 1A and 3A might be new adult-plant resistance QTLs to w heat leaf rust ,w hich enriched the w heat slow rusting gene pool ,and their closely linked mak-ers could be used for maker-assisted breeding of durable resistant cultivars .

关 键 词：小麦叶锈病 成株慢锈基因 QTL作图 

分 类 号：S435.121.43[农业科学—农业昆虫与害虫防治]