瘦素减轻鱼藤酮诱导的SH-SY5Y细胞损伤  

Leptin alleviates rotenone-induced injury of SH-SY5Y cells

作　　者：韩铭[1] 张金英[1] 宋翠红[1] 梁辰[1] 冯杰[1] 颜光涛[1] 

机构地区：[1]解放军总医院基础所生化室,北京100853

出　　处：《解放军医学院学报》2014年第2期170-173,共4页Academic Journal of Chinese PLA Medical School

基　　金：科技基础性工作专项项目(2011FY130100);国家科技支撑计划项目(2012BAK25B01)~~

摘　　要：目的探讨瘦素对鱼藤酮(rotenone)所诱导的人神经母细胞瘤SH-SY5Y细胞凋亡的抑制作用。方法建立鱼藤酮诱导的SH-SY5Y细胞损伤模型,用瘦素进行干预,采用MTT比色法检测细胞存活率,锥虫蓝检测细胞死亡率,细胞形态学观察,并用Western blot法检测Bcl-2、Bax、p-GSK-3β(Ser9)和GSK-3β的表达水平,免疫荧光检测α-synuclein的表达水平。结果成功建立了鱼藤酮诱导的SH-SY5Y细胞损伤模型。在模型的基础上,瘦素干预后,细胞状态改善,细胞存活率提高约14%(P<0.05),细胞死亡率降低约17%(P<0.05),α-synuclein的浓度下降,Bcl-2和p-GSK-3β的表达显著提高。结论瘦素对鱼藤酮诱导的SH-SY5Y细胞损伤具有明显的抑制作用,可能是通过抑制GSK-3β的活性上调Bcl-2/Bax的比率和降低α-synuclein的表达而得以实现。Objective To study the inhibitory effect of leptin on rotenone-induced apoptosis of human neuroblastoma SH-SY5Y cells. Methods A SH-SY5Y cell injury model was induced by rotenone and intervened with leptin. Viability of SH-SY5Y cells was assayed by MTT, apoptosis of SH-SY5Y cells was detected with trypan blue staining, morphology of SH-SY5Y cells was observed under inverted microscope, expression levels of Bcl-2, Bax, p-GSK-3 β （Ser9）, GSK-3 β and α -synuclein were measured by Western blot and immunofluorescence, respectively. Results The rotenone-induced SH-SY5Y cell injury model was successfully established. The survival rate of SH-SY5Y cells was about 14% higher（P 〈 0.05）, the apoptosis rate of SH-SY5Y cells was about 17% lower（P 〈 0.05）, the α -synuclein expression level was significantly lower whereas Bcl-2 and p-GSK-3 β expression was significantly higher after leptin intervention than before leptin intervention. Conclusion Leptin can significantly inhibit rotenoneinduced SH-SY5Y cell injury by up-regulating the Bcl-2/Bax ratio and down-regulating the expression of α -synuclein through the activity of GSK-3 β .

关 键 词：鱼藤酮 SH—SY5Y细胞 瘦素 

分 类 号：R34[医药卫生—基础医学]