3个美洲黑杨新无性系的SSR分析及鉴定  

Analysis and Identification of Three New Clones of Populus deltoides Based on SSR Molecular Markers

作　　者：李薇[1] 刘春英[1] 郭娟[1] 王永进 樊军锋[1] 

机构地区：[1]西北农林科技大学林学院,陕西杨陵712100 [2]岚皋县林业技术推广服务中心,陕西岚皋725400

出　　处：《西北林学院学报》2014年第1期73-77,共5页Journal of Northwest Forestry University

基　　金："十二五"农村领域国家科技计划专题"超高产优质杨树速生林新品种选育"(2012BAD01B03)

摘　　要：利用SSR-PCR的方法对6个美洲黑杨(Populus deltoides)无性系进行了基因组多态性分析。从30对引物中筛选出10对特异性引物并进行PCR扩增,共扩增出了132个位点,其中多态性位点125个,所占比率为94.69%,基因型间的平均遗传距离为0.45。聚类分析结果表明,各杨树无性系间存在一定的遗传差异,准确的反映了遗传差异对外部形态的反应。Simple sequence repeat（SSR）was used to determine the genomic DNA variations and genetic relationships in Populus deltoides.Six cultivars were used in SSR analysis.A total of 10SSR primers which were polymorphic and informative were selected to determine the genetic diversity and genetic relationships.One hundred and thirty two DNA bands were amplified,125 of which were polymorphic（94.69%）. The average genetic distance between genotypes was 0.45.The cluster analysis demonstrated the existence of genetic differences between varieties of Populus,accurately reflecting the genetic differences in response to the external morphology of the reaction.

关 键 词：美洲黑杨 SSR 品种鉴定 遗传多态性 

分 类 号：S792.119[农业科学—林木遗传育种]