体外心肌细胞缺血后适应模型的建立及评价  

The establishment and evaluation of vitro ischemic postconditioning modle of myocardial cells

作　　者：李晓梅[1] 包馨慧 杨毅宁[1] 马依彤[1] 陈邦党[1] 刘芬[1] 李海霞[1] 

机构地区：[1]新疆医科大学第一附属医院心脏中心,乌鲁木齐830054

出　　处：《新疆医科大学学报》2014年第1期24-28,共5页Journal of Xinjiang Medical University

基　　金：国家自然科学基金(81060021);新疆维吾尔自治区高校科研计划科学研究重点项目(XJEDU2010I129);高等学校博士学科点专向科研基金(2012211A062);新疆医科大学第一附属医院科研奖励基金(2010YFY07)

摘　　要：目的建立心肌细胞缺血后适应(IPost)模型并观察IPost对心肌细胞再灌注(I/R)损伤的影响。方法体外培养新生SD乳鼠心肌细胞,随机分正常对照组(SH组)、缺血再灌注组(I/R组)及缺血后适应组(IPost组),用烟酸己可碱(Hochest33342)及碘化丙啶混合溶液染色后观察细胞凋亡率情况,流式细胞术检测细胞凋亡率,逆转录PCR(RT-PCR)方法和蛋白质印迹法(Western Blot)方法检测Bcl-2/Bax基因表达。结果与I/R组比较,IPost组细胞凋亡率显著改善,Bcl-2蛋白表达上调,Bax蛋白表达下降,Bcl-2/Bax显著增加(P均<0.05)。结论 IPost能通过抗心肌凋亡有效地减轻离体心肌细胞缺血再灌注损伤。Objective To evaluate the effect of ischemic postconditioning （IPost） on ischemia reperfusion injury by establishing the Ipost modle of myocardial cells. Methods Myocardial cells of SD neonatals were cultured in vitro, and divided randomly into 3 groups： SH group, I/R group and IPost group. The morphology of myocardial cells was cultured through inverted fluorescence microscope. The cells were stained by Hochest33342 to observe the cell＇s apoptosis situation. Flow cytometry was adopted to evaluate the apoptosis rate of cells; RT-PCR and Western Blot were used to test the mRNA of Bcl-2/Bax. Results Compared with the I/R group, the apoptosis rate in IPost group was greatly improved, the expression of Bcl-2 was upregulated while Bax downregulated, and Bcl 2/Bax increased （P 〈0.05）. Conclusion IPost can protect the myocardiol cells in vivo from ischemia reperfusion injury by opposing to the apoptosis of myocardiol cells.

关 键 词：缺血后适应 心肌缺血 再灌注损伤 心肌细胞 

分 类 号：Q463[生物学—生理学]