推拿对坐骨神经损伤大鼠NT-3、TrkC的影响  

Tuina's Effect on NT-3/TrkC of Sciatic Nerve Injury Rats

作　　者：耿楠[1] 冼思彤 吴剑聪[1] 鲁梦倩[1] 姚斌彬[1] 高玉峰[1] 李小琴[1] 于跃[1] 于天源[1] 

机构地区：[1]北京中医药大学针灸推拿学院,北京100029

出　　处：《南京中医药大学学报》2014年第1期46-49,共4页Journal of Nanjing University of Traditional Chinese Medicine

基　　金：国家自然科学基金(30873311);北京中医药大学在读研究生资助项目

摘　　要：目的研究推拿对坐骨神经损伤大鼠感觉功能及NT-3、TrkC表达的影响。方法采用夹持法建立坐骨神经损伤模型,将大鼠随机分为正常组、假手术组、模型组、模型对照组、推拿组,以按摩推拿手法模拟仪进行干预,通过热痛阈实验观察各组大鼠行为学变化;通过免疫组织化学染色观察各组大鼠背根节内NT-3、TrkC表达情况。结果模型组、模型对照组大鼠的PWL值与正常组相比明显延长,推拿组与模型组相比PWL值明显缩短,与正常组相比无显著差异。模型组、模型对照组及推拿组NT-3免疫组化表达与正常组比较均有明显提高,推拿组与模型组比较有显著性差异(P<0.05)。结论中医推拿可以改善神经损伤大鼠的行为学表现,通过促进NT-3及其受体TrkC的表达,发挥抑制细胞凋亡,对神经元胞体和突起的生长产生积极的影响,促进损伤神经的修复,改善坐骨神经损伤大鼠的感觉功能。OBJECTIVE To study the Tuina＇s effect on the sensory function of sciatic nerve injury rats and the NT-3/TrkC expression. METHODS Using the chucking method to establish the sciatic nerve injury model and the rats were randomly divided into normal group, sham-operated group, model group, model control group and the Tuina group. Imulator was used as intervention to observe the rats be havior change. Immunohistochemical staining was used to observe the expression of each rat dorsal root section in the NT-3 TrkC. RESULTS Compared with the normal group, the model group, model control group rats＂ PWL value were significantly extended. Compared with model group, the Tuina group＇s PWL value reduced and no signifi- cant difference compared with normal group. Compared with the normal group, model group, model control group and the Tuina group＇s NT-3 immunohistochemical expression were increased significantly. Compared with model group, control group has significant difference （Pal0.05）. CONCLUSION TCM Tuina can improve the behavior performance of nerve injury in rats. By promoting the expression of the NT-3 and the receptor TrkC, cell apoptosis were inhibited; the growth of nerve cell body and processes were influenced, which have a positive impact on the damage of nerve repair and the improvement of motor function of sciatic nerve injury in rats.

关 键 词：推拿 坐骨神经损伤 热痛阈实验 NT-3 TRKC 

分 类 号：R244.1[医药卫生—针灸推拿学]