HPLC同时测定复方五味含片中葛根素、甘草苷、甘草酸铵、五味子醇甲、五味子甲素、五味子乙素  

Simultaneous Determination of Six Components in Compound Wuwei Buccal Tablets by HPLC

作　　者：单鑫[1] 陆兔林[1] 毛春芹[1] 季德[1] 郎永英 黄双[1] 

机构地区：[1]南京中医药大学药学院,江苏南京210023

出　　处：《南京中医药大学学报》2014年第1期82-84,共3页Journal of Nanjing University of Traditional Chinese Medicine

基　　金：国家科技重大专项项目(2009ZX09308-004)

摘　　要：目的建立HPLC同时测定复方五味含片中葛根素、甘草苷、甘草酸铵、五味子醇甲、五味子甲素、五味子乙素6种成分含量的方法。方法KromasiL C18柱（250mm×4．6mm，5μm）；流动相：乙腈－0．05％磷酸水溶液，梯度洗脱；流速1．0mL／min；柱温30℃，检测波长250nm。结果葛根素、甘草苷、甘草酸铵、五味子醇甲、五味子甲素、五味子乙素分别在0．0310～0．1549、0．0137～0．0685、0．0424～0．2118、0．0174～0．0871、0．0018～0．0091、0．0136～0．0681mg／mL线性范围良好；相关系数均大于0．9995；平均加样回收率分别为98．6％、100．4％、98．3％、98．3％、96．8％、99．4％；RSD分别为2．6％、2．8％、1．2％、2．3％、0．9％、2．5％。结论该方法简便、重复性好，可用于复方五味含片的质量控制。OBJECTIVE To establish a method to simultaneously determine puerarin, liquiritin, ammonium glycyrrhetate, schizandrol, deoxyschisandrin, and schizandrin B in compound wuwei tablet by HPLC. METHODS The elusion was performed on Kromasil C18 （250mm×4.6mm,5μm） column. The mobile phase was composed of acetonitrile and 0.05 % H3 PO4 with gradient elution. The detection wave length was 250 nm, the flow rate was 1.0 mL/min and the column temperature was maintained at 30 ℃. RESULTS Puerarin, liquiritin, ammonium glyeyrrhetate, schizandrol, deoxyschisandrin, schizandrin B are all linear in the range of 0.031 0-0. 154 9,0.013 7-0.068 5,0.042 4-0.211 8,0.017 4-0.087 1,0.001 8-0.009 1,0.013 6-0.068 1 mg/mL respectively. Their average recoveries were 98.6%, 100.4%,98.3%,98.3%,96.8%,99.4% and RSD of the above six components were 2.6 %, 2.8 %, 1.2 %, 2.3 %, 0.9 %, 2.5 %. CONCLUSION The method is easy to perform and accurate and practical so that it can be used to determine the six components in the compound wuwei buccal tablets.

关 键 词：复方五味含片 葛根素 甘草苷 甘草酸铵 五味子醇甲 五味子甲素 五味子乙素 

分 类 号：R284.1[医药卫生—中药学]