橡胶树胶乳橡胶粒子死皮相关蛋白的鉴定及分析  

Identification and analysis of latex rubber particle proteins related with tapping panel dryness in Hevea brasiliensis

作　　者：袁坤[1] 周雪梅[1,2] 王真辉[1] 杨礼富[1] 

机构地区：[1]中国热带农业科学院橡胶研究所,农业部橡胶树生物学与遗传资源利用重点实验室,海南儋州571737 [2]海南大学农学院,海南海口570100

出　　处：《南京林业大学学报（自然科学版）》2014年第1期36-40,共5页Journal of Nanjing Forestry University：Natural Sciences Edition

基　　金：中央级公益性科研院所基本业务费专项项目(YWFZX2008-12,2009-12,2010-9,1630022013003);海南省重点科技项目(90107);现代农业产业技术体系建设专项资金项目(CARS-34-02A)

摘　　要：橡胶树死皮是影响天然橡胶产量的重要因子之一。采用双向凝胶电泳对健康树和死皮树橡胶粒子中差异表达的蛋白进行分离,分析获得35个差异表达的蛋白点,通过基质辅助激光解析电离飞行时间质谱(MALDITOF MS)分析及搜索Uniprot rubber数据库后,有13个蛋白点被成功鉴定,其中橡胶延长因子(rubber elongation factor,REF)、法尼基焦磷酸合成酶(Ffarnesyl-diphosphate synthase,FPS)、谷胱甘肽过氧化酶(glutathione peroxidase,GPX)、谷胱甘肽还原酶(glutathione reductase,GR)、翻译控制肿瘤蛋白(translationally controlled tumor protein,TCTP)、热激蛋白(heat shock protein,HSP)等主要蛋白参与了橡胶的生物合成、活性氧代谢及细胞凋亡过程。说明橡胶的生物合成、活性氧代谢及细胞凋亡途径可能是橡胶树死皮发生的关键调控途径。The tapping panel dryness （TPD） in Hevea brasiliensis is one of the important factors influencing natural rub- ber production. In order to distinguish and identify the differentially expressed proteins of latex rubber particles in healthy and TPD trees, the two-dimensional electrophoresis （2-DE） was used. 35 differentially expressed protein spots were ob- tained by software analysis. Subjecting to MALDI-TOF MS following by searching for Uniprot rubber database, 13 protein spots were successfully identified, Of them, REF, FPS, GPX, GR, TCTP and HSP were mainly involed in the rubber biosynthesis, reactive oxygen metabolism and cell apoptosis processes. This study further showed that the pathways of the rubber biosynthesis, reactive oxygen metabolism and cell apoptosis were possibly the key regulated ones of TPD and which would provide a theoretical basis for elucidating the TPD mechanism in Hevea brasiliensis.

关 键 词：橡胶树死皮 橡胶粒子 差异表达蛋白 双向凝胶电泳 质谱 

分 类 号：S794.1[农业科学—林木遗传育种]