紫丁香叶片发育过程中花色素苷含量与叶绿素荧光和激发能分配的关系  

The relationship between leaf anthocyanin content and chlorophyll fluorescence,as well as excited energy distribution during leaf expansion of Syringa oblata Lindl.

作　　者：田野[1] 张会慧[1] 张秀丽[1] 王娟[1] 齐飞[1] 孙广玉[1] 

机构地区：[1]东北林业大学生命科学学院,黑龙江哈尔滨150040

出　　处：《南京林业大学学报（自然科学版）》2014年第1期59-64,共6页Journal of Nanjing Forestry University：Natural Sciences Edition

基　　金："十二五"国家科技支撑计划(2011BAD08B02-3);国家自然科学基金面上项目(31070307;30771746);黑龙江省自然科学基金重点项目(ZD201105)

摘　　要：以紫丁香(Syringa oblata Lindl.)为试验材料,研究了叶片展开过程中花色素苷含量变化对叶绿素含量、叶绿素荧光特性和光合激发能分配的影响。结果表明:紫丁香叶片从幼叶伸出到叶片成熟的各生育阶段,花色素苷含量降低,而叶绿素含量增加,二者之间呈明显的负相关,叶片对光能的捕获和利用能力也随着花色素苷的降低而增强,但是,从叶片伸出到叶片展开,叶片最大光化学效率(F v/F m)没有明显变化。在紫丁香叶片伸出到扩展的进程中,花色素苷含量降低,PSⅡ反应中心吸收的光能分配到失活PSⅡ反应中心的热耗散量子产额(ФNF)下降,以及依赖于叶黄素循环的量子产额(ФNPQ)下降。说明叶片叶龄越小,花色素苷含量越多,依赖于类囊体膜两侧质子梯度和叶黄素循环耗散的能量比例明显高于成熟叶片,而且PSⅡ反应中心吸收光能分配到无活性反应中心的比例较大,这种光能的分配机制明显降低了幼叶中有活性PSⅡ反应中心的压力,因此,花色素苷可防止幼叶光合机制受到过剩光能的破坏。In this paper, effects of changes in anthayanin content on chlorophyll contents, chlorophyll fluorescence char- acteristics and excitation energy distribution in photosynthetic apparatus in leaves of Syringa oblata Lindl.during leaf ex- pansion were investigated. The results showed that anthoeyanin contents decreased and chlorophyll contents increased in leaves of S. oblata with the expansion of leaves. There was a significant negative relationship between anthocyanin content and chlorophyll content, and capability of light capture and utilization of leaves were strengthened gradually with the in- creasing of anthocyanin contents, but the maximal PS I1 photochemical efficiency （ Fv/Fm ） had no significant change. During leaf expansion, leaf anthocyanin contents decreased, and the quantum yield of thermal dissipation in non-func- tional PS II （ΦNF） and the quantum yield of light-dependent and /k pH-and xanthophyll-mediated regulated thermal dis- sipation （ ΦNPQ ） decreased. This showed that the younger leaf age was, the more anthocyanin contents had. And not only energy proportion of trans-thylakoid pH gradient and xanthophyll-regulated thermal energy dissipation in young leaves were higher than in mature leaves, but also quantum yield of thermal dissipation assigned by light absorption of PS Ⅱ re- action center to non-functional PS Ⅱwas larger. The distribution mechanism of light energy significantly decreased the pressure of active PS II response center in young leaves, therefore, anthocyanin can prevent young leaves form the de- struction of excess light energy.

关 键 词：紫丁香 花色素苷 叶绿素荧光 激发能 

分 类 号：Q945.1[生物学—植物学]