基于DNA的木材树种识别研究进展  

Progress of wood identification based on DNA methods

作　　者：张蓉[1,2] 殷亚方[2] 徐魁梧[1] 叶克林[2,1] 

机构地区：[1]南京林业大学材料科学与工程学院,江苏南京210037 [2]中国林业科学研究院木材工业研究所,北京100091

出　　处：《南京林业大学学报（自然科学版）》2014年第1期151-156,共6页Journal of Nanjing Forestry University：Natural Sciences Edition

基　　金：国家林业公益性行业科研专项项目(201304508);江苏高校优势学科建设工程资助项目(PAPD)

摘　　要：从木材识别的角度,对木材DNA提取方法(CTAB、SDS、PTB、DNeasy Plant Mini Kit等)和DNA条形码及DNA指纹图谱等基于DNA的木材树种识别方法进行了综合评述。其中,针对DNA条形码方法,重点阐述了基因组DNA中可用的特征序列来源叶绿体基因(chloroplast DNA,cpDNA)rbcL、matK、trnH-psbA间隔区序列,核糖体基因(ribosomal DNA,rDNA)ITS序列,以及变异位点的识别和系统进化树的应用等问题;针对DNA指纹图谱方法,重点阐述了(RAPD、ISSR、SSR、SNP)4种DNA分子标记方法在DNA指纹图谱中的研究和应用现状。笔者认为,以DNA特征序列为依据的木材树种识别理论上虽然是可行的,但要应用于实际还需开展更多的研究。From wood identification points of view, DNA extraction methods （ CTAB, SDS, PTB, DNeasy Plant Mini Kit etc. ） and DNA barcode and fingerprint and wood identification methods based on DNA were summarized. For the DNA barcode concerned, the emphases were on the signature sequences in genome DNA （chloroplast DNA, cpDNAr- bcL, matK,trnH-psbAintergenic spacer sequences, and ribosomal DNA, rDNA ITS）as well as identification form （varia- ble sites and phylogenetic tree） described. For the DNA fingerprint method,the emphases were on the progress and out- look of 4 kinds of molecular makers such as random amplificated polymorphism DNA, RAPD,inter-simple sequence re- peat, ISSR ,simple sequence repeats, SSR ,single nucleotide polymorphism, SNP. It was concluded that wood identifica- tion based on DNA methods is feasible in theor＇ but needs more studied on.

关 键 词：木材树种识别 DNA提取 DNA条形码 DNA指纹图谱 

分 类 号：S781[农业科学—木材科学与技术]