枣实蝇及其他5种检疫性实蝇的PCR-RFLP快速鉴定  

Rapid identification of Carpomya vesuviana Costa and five relative species of fruit flies by PCR-RFLP

作　　者：程晓甜[1,2] 阿地力·沙塔尔[1,2] 张伟[3] 李新泉[1,2] 

机构地区：[1]新疆农业大学林学与园艺学院,新疆乌鲁木齐830052 [2]新疆农业大学,新疆教育厅干旱区林业生态与产业技术重点实验室,新疆乌鲁木齐830052 [3]新疆出入境检验检疫局技术中心,新疆乌鲁木齐830083

出　　处：《南京林业大学学报（自然科学版）》2014年第1期183-185,共3页Journal of Nanjing Forestry University：Natural Sciences Edition

基　　金：新疆维吾尔自治区科技计划项目(201130102-3);新疆维吾尔自治区重点学科森林培育科研项目

摘　　要：枣实蝇(Carpomya vesuviana Costa)是一种新入侵检疫性有害生物。应用PCR-RFLP技术,快速区分鉴定了枣实蝇与我国口岸截获频率较高的5种检疫性实蝇。研究表明:设计出的2对引物对6种供试实蝇线粒体DNA(mtDNA)进行PCR扩增,其扩增片断大小分别为350 bp和450 bp。PCR扩增产物用2种限制性内切酶Dra I和MseI进行酶切,根据不同的酶切位点准确区分了6种供试的实蝇。此方法可用于口岸截获实蝇的快速检疫鉴定。Carpomya vesuviana Costa has been a new quarantine pest in China. In this paper, the method of rapid identi- fication of C. vesuviana Costa and 5 relative species of fruit flies was studied based on PCR-RFLP of the mitochondrial DNA（mtDNA）. The results showed that DNAs, which were estimated about 350 bp and 450 bp respectively, were ob- tained by means of PCR with two pairs of primers designed from mtDNA of target pests.The PCR-products could be reg- istered by restriction enzymes （Dra I and Mse I）. All the 6 species could be discriminated according to the banding pat- terns of the restriction enzymes. It is suggested that the method is reliable for quick discrimination of fruit flies.

关 键 词：枣实蝇 检疫性实蝇 PCR-RFLP 线粒体DNA 

分 类 号：Q965[生物学—昆虫学]