种子超干贮藏对紫花苜蓿前期生长和生理特性的影响  

Effects of seed ultra-drying storage on growth and physiological characteristics of Medicago sativa seedlings

作　　者：霍平慧[1,2] 李剑峰[1,2] 师尚礼[1,2] 张淑卿[1,2] 

机构地区：[1]甘肃农业大学草业学院,甘肃兰州730070 [2]草业生态系统教育部重点实验室/中-美草地畜牧业可持续发展研究中心,甘肃兰州730070

出　　处：《干旱地区农业研究》2014年第1期171-176,267,共7页Agricultural Research in the Arid Areas

基　　金：国家自然科学基金(31060326);甘肃农业大学盛彤笙科技创新基金(GSAU-STS-1224)

摘　　要：采用硅胶室温干燥法对陇东紫花苜蓿（Medicago sativa L. cv. Longdong）种子进行超干处理，使其含水量由9.03%（CK）分别降至7.09%～4.59%共八个不同含水量水平，用铝箔纸密封，常温下贮藏1年后，采用沙培盆栽试验，研究种子超干贮藏对苜蓿生长和生理的影响。结果表明，超干贮藏后，紫花苜蓿种子的出苗数、植株的株高比CK显著提高，MDA含量显著降低。播种第30天时，含水量为6.36%～4.59%处理种子的出苗数较CK提高25%～50%；播种第60天时，种子含水量为6.36%～5.46%处理的植株株高较CK提高11.02%～15.68%，各超干处理种子的植株MDA含量仅为CK的44.92%～70.29%；所有处理植株的根体积、地上干重、地下干重和可溶性糖含量仅为CK的55.36%～89.29%、63.06%～88.05%、50.71%～76.69%和63.35%～97.86%；除4.59%含水量处理的叶片数和5.18%含水量处理的根瘤数与CK比差异不显著，其它各处理的单株叶片数与根瘤数均显著低于CK，其中各超干处理的根瘤数仅为CK的63.00%～92.56%。说明超干贮藏能够提高苜蓿种子的出苗数、植株的株高和耐受性，但降低了植株的生物量及根体积，并影响了叶片生长及根瘤的形成。Medicago sativa L. cv. Longdong was used as test material to explore the effect of ultra-drying storage on seedling growth. The seeds were ultra-dried from an initial moisture content of 9.03% （CK） to 7.09%～4.59%, respectively, with silica gel method, and then hermetically sealed in aluminized paper at ambient temperature for storage. One year later, all the seeds with different moisture contents were sown in pot with sand to study the growth and physiology of the resulting seedlings. The result indicated that moderately ultra-dried seeds performed better in seedling emergence, shoot height and MDA content, on day 30, seedling number of treatments with moisture contents from 6.36 to 4.59 were 25%～50% times higher than the control, and on day 60, shoo t height of treatments with moisture contents from 6.36 to 5.46 were 11.02%～15.68% times higher than control. MDA content of all the ultra-drying treatments were only 44.92%～70.29% of the CK treatment. Root volume, aboveground dry weight, underground dry weight and soluble sugar content were only 55.36%～89.29%, 63.06%～88.05%, 50.71%～76.69% and 63.35%～97.86% of the CK, respectively, ex cept the non-significant difference of 4.59% treatment in leaf number and 5.18% treatments in nodule number compared with CK All the other treatments were found significantly lower than CK in leaf and nodule numbers, and the nodule number of the ultra-dry treatments was only 63.00%～92.56% of CK, indicating that ultra-drying storage could increase seedling emergence, shoot height and plant resi stance, but decrease plant biomass and root volume.

关 键 词：紫花苜蓿 种子超干处理 幼苗生长 生理 

分 类 号：S330.31[农业科学—作物遗传育种] Q945.78[农业科学—农艺学]