徐薯55-2的胚性细胞悬浮培养  

Embryogenic Suspension Culture of Xushu 55-2

作　　者：王欣[1,2] 闫会[1] 顾向华[2] 马代夫[1,2] 李强[1,2] 

机构地区：[1]中国农业科学院甘薯研究所,甘薯遗传改良重点开放实验室,江苏徐州221131 [2]江苏徐州甘薯研究中心,农业部甘薯生物学与遗传育种重点实验室,江苏徐州221131

出　　处：《西北农业学报》2014年第1期132-137,共6页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：国家高技术研究发展计划(“863”计划);现代农业产业技术体系建设专项资金(CARS-11)

摘　　要：为优化胚性愈伤的培养条件,建立甘薯高效胚性悬浮培养试验操作体系,以徐薯55-2、徐薯18、徐薯22、徐薯28、徐薯29和徐紫薯5号等6个甘薯品种(系)茎尖诱导的胚性愈伤组织为材料,通过比较不同品种的胚性愈伤组织诱导率、愈伤状态等的差异,筛选出愈伤诱导率高,愈伤状态好,体胚诱导率及成苗率高的品种徐薯55-2。通过测定细胞沉降体积(SCV)研究徐薯55-2胚性细胞悬浮系的接种密度、接种量、摇床转速对胚性悬浮细胞生长的影响。结果表明,徐薯55-2胚性细胞悬浮系适宜培养条件为接种密度1∶25,接种量1.4mL,摇床转速100r/min。Embryogenic calli induced from shoot apices of 6 cultivars of sweetpotato Elpornoea batatas （L）. Lam.-1, including Xushu 18, Xushu 22, Xushu 28, Xushu 29, Xushu 55-2, and Xuzishu 5 were used to optimize the culturing conditions of embryogenic callus and establish efficient experiment operating system of sweetpotato embryogenic suspension culture. Xushu 55-2 performed better with high efficiency, good callus status, high somatic embryogenesis and plant regeneration rate compared with other cultivars. Affecting factors of embryogenic suspension callus of Xushu 55-2 were studied by measuring its sedimented cell volume （SCV）. The results developed the optimal culture conditions of embryogenic cell suspension. The inoculation density was 1 ： 25, inoculation quantity was 1.4 mL, rotation speed was 100 r/min.

关 键 词：徐薯55—2 SCV 胚性细胞悬浮系 影响因素 

分 类 号：S531[农业科学—作物学]