白细胞介素-2及其受体与乙型肝炎疫苗接种无、弱应答发生的关系  

Relationship between interleukin-2, interleukin-2 receptor and the non-and hypo-responsiveness to hepatitis B vaccine

作　　者：谢婧[1] 王学良[1] 庄贵华[1] 徐慧文[1] 郝炳华[1] 唐晓红 吴谦 

机构地区：[1]西安医科大学流行病学教研室,西安710061

出　　处：《中华肝脏病杂志》2000年第6期332-334,共3页Chinese Journal of Hepatology

摘　　要：目的：探讨白细胞介素-2（IL-2）与可溶性白细胞介素-2受体（sIL-2R）与乙型肝炎疫苗接种无、弱应答的关系。 方法：在HBsAg和植物血凝素（phytohaemagglutinin，PHA）两种刺激条件下，对21名乙型肝炎疫苗接种无、弱应答者、22名强应答者和21名HBsAg慢性携带者的外周血单个核细胞进行体外培养，并用酶联免疫法测定细胞培养上清液中IL-2和sIL-2R的水平。 结果：无、弱应答组的IL-2水平明显低于强应答组（t＝8.80，P＜0.001＝，而与HBsAg慢性携带组相近（q＝0.06, P＞0.5）。无、弱应答组和强应答组的sIL-2R水平差异无显著意义。结论：外周血单个核细胞分泌IL-2 水平低下可能是乙型肝炎疫苗接种后无、弱应答发生的原因之一。Objective：To study the relationship between interleukin-2 (IL-2), soluble interleukin-2 receptor (sIL-2R) and the non-and-hypo-responsiveness to hepatitis B vaccine. Methods： Peripheral blood mononuclear cells (PBMC) isolated from 21 non-and -hypo-responders, 22 hyperresponders and 21 chronic HBsAg carriers were incubated in the presence of HBsAg and phytohaemagglutinin (PHA). The levels of IL-2 and sIL-2R in the supernatants of activated cells were measured by an enzyme-linked immunosorbent assay. Results：The average level of IL-2 in the non-and-hypo-responders was significantly lower than that in the hyperresponders (t=8.80, P<0.001), but was comparable to that in chronic HBsAg carriers (q=0.06, P>0.5). Between the hyperresponders and the non-and-hypo-responders, the average sIL-2R levels showed no noticeable difference. Conclusion：The results suggest that low level of IL-2 may be one of the causes and mechanisms of non-and-hypo-responsiveness to hepatitis B vaccine.

关 键 词：乙型肝炎疫苗 白细胞介素-2 IL-2 SIL-2R 乙型肝 

分 类 号：R512.62[医药卫生—内科学]