乙型肝炎患者外周血单个核细胞IL-6 mRNA表达的定量分析  

A quantitative analysis of IL-6 mRNA expression of peripheral blood monocyte cell in patients with chronic hepatitis B

作　　者：宋卫东[1] 张奉学[2] 李振纲 

机构地区：[1]中国科学技术大学生命科学院,合肥230028 [2]广州中医药大学热带医学研究所

出　　处：《中华肝脏病杂志》2000年第6期346-347,共2页Chinese Journal of Hepatology

摘　　要：目的：探讨IL-6参与乙型肝炎的免疫病理损伤和病毒清除的机理。 方法：采用ELISA法检定了慢性乙型肝炎（chronic hepatitis B，CHB）患者血清IL-6含量，竞争性RT-PCR-ELISA法分析了CHB外周血单个核细胞的IL-6 mRNA表达水平。 结果：CHB血清IL-6含量明显升高，竞争性RT-PCR结果表明对照组和患者组IL-6 mRNA靶片段表达频率分别为41.7%（5/12）和82.1%（23/28），定量ELISA表明5例对照组IL-6 mRNA 拷贝数在1000～10000之间，平均拷贝数的对数为3.65；而患者组均大于10000，平均拷贝数的对数为4.40。结论： IL-6在乙型肝炎发病中具有重要作用，而IL-6水平升高主要与患者外周血单个核细胞（peripheral blood monocyte cell，PBMC）IL-6 mRNA表达增强有关。Objectives: To investigate the relationship of interleukin-6 (IL-6) mRNA expression with the immunopathogenesis and viral clearance. Methods: The serum IL-6 level of patients with chronic hepatitis B (CHB) were detected by ELISA and quantitatively the IL-6 mRNA expression of peripheral blood monocyte cell (PBMC) in patients with CHB were analysed by competitive RT-PCR-ELISA. Results: Serum level of IL-6 in patients with CHB was significantly increased. Both expression rates of IL-6 mRNA target fragment in control and in patients with CHB were 41.7% (5/12) and 82.1% (23/28) respectively. The quantitative ELISA of production of RT-PCR showed the copies of IL-6 mRNA in 5 cases of control were 1000 to 10000 (the mean log copies was 3.65), while the copies of IL-6 mRNA in 23 cases of patients were all more then 10000 (the mean log copies was 4.40). Conclusion: This study shows that the increased level of IL-6 may play some role in hepatitis B infection, the increased serum level of IL-6 is related to the enhanced expression of IL-6 mRNA of PBMC.

关 键 词：白细胞介素-6 MRNA 慢性乙型肝炎 

分 类 号：R512.620.2[医药卫生—内科学]