脂多糖致小鼠SIRS和MODS的研究  

Study on SIRS and MODS induced by lipopolysaccharide

作　　者：魏育林[1] 李亚俊[1] 罗乃丹[1] 刘轩[1] 戴书静[1] 金恩源[1] 

机构地区：[1]中日友好医院临床医学研究所,北京100029

出　　处：《急诊医学》2000年第6期370-373,共4页

基　　金：国家劳动社会保障部非教育系统留学回国人员资助项目!(人发 [1998]87号文件 )

摘　　要：目的 :观察腹腔注射脂多糖诱导小鼠内毒素性全身炎症反应综合征和多器官功能不全综合征的作用。方法 :选用 5～ 7周雄性BALB/c小鼠 ,腹腔注射不同剂量大肠杆菌脂多糖 ,观察小鼠一般状态、死亡率、肛温及脏器的病理变化 ,并重点观察了 6mg/kgLPS诱导小鼠TNF α分泌的作用及其对小鼠代谢和器官功能的影响。结果 :腹腔注射LPS可致小鼠出现腹泻、紫绀、竖毛等症状 ,肛温和死亡率变化呈LPS剂量依赖性。LPS对小鼠的半数致死量为 9 0mg/kg。 6 0mg/kg的LPS剂量可使小鼠代谢紊乱 ,表现为血糖降低 ,血脂及载脂蛋白水平升高 ;可使肺、小肠、心、肾、肝等器官损伤 ,表现为血清胆红素、谷草转氨酶、磷酸肌酶、尿酸水平升高 ,病理形态学表现为充血、水肿、炎细胞浸润甚至细胞坏死。受累的脏器数随LPS剂量的增加而增加。结论 :腹腔注射LPS (6 0mg/kg)可致小鼠SIRS和MODS ,9 0mg/kg体重LPS为其半数致死剂量。Objective:To investigate the changes of SIRS and MODS induced by intraperitoneal injection of LPS in mice.Methods:BALB/c mice (male,5～7 weeks old)were injected intraperitoneally with different doses of LPS(E.Coli 0111: B4,Sigma).The rectal temperature,mortality rate and pathological changes in the thymus,spleen,lung,heart,kidney,small intestine,liver and brain were examined.Using biochemical method,the white blood cell count,the metabolism and the function of organs in mice treated with 6 0mg/kg LPS were also observed.Endotoxin concentration and TNF α were measured by Limulus test and ELISA,respectively.Results:6 0mg/kg LPS decreased the concentration of glucose and increased concentration of BIL,CPK,UA,GOT/GPT in serum of the mice;ard also impaired the functions of small intestine,heart,kidney and liver.9 0 mg/kg LPS killed 50% of the mice,while 24 0mg/kg LPS killed all of the mice.Conclusion:6 0mg/kg LPS can induce SIRS and MODS in mouse.

关 键 词：内毒素 多器官衰竭 脂多糖 小鼠 SIRS MODS 

分 类 号：R595.3[医药卫生—内科学]