血液净化材料在体外静态接触血浆时血浆中C3a des Arg生成的研究  

In Vitro Study of C3a des Arg Produced by Plasma Incubation with Blood Purification Materials

作　　者：黄嘉[1] 程莉萍 乐以伦[1] 魏启欧[2] 

机构地区：[1]四川大学高分子材料系 [2]华西医科大学附属第一医院移植免疫实验室,成都610041

出　　处：《生物医学工程学杂志》2000年第4期385-389,共5页Journal of Biomedical Engineering

摘　　要：用放射免疫分析法研究了五种血液净化材料 :磺化聚醚砜 ,聚醚砜 ,聚砜 ,聚甲基丙烯酸甲酯和醋酸纤维素与人血浆在 37℃温育 30、6 0、90、12 0min后 ,血浆中补体C3激活产物C3adesArg的生成情况。结果发现 :醋酸纤维素、聚甲基丙烯酸甲酯、聚醚砜、聚砜在与血浆温育后 ,血浆中C3adesArg的浓度依次降低 ;以醋酸纤维素激活补体C3的情况最严重 ;磺化聚醚砜的最低 ,并且磺化度越大 ,C3adesArg的浓度越小。说明聚醚砜材料作为血液净化材料有较优异的补体相容性 ,并且通过磺化可大大提高聚醚砜的补体相容性。放免分析法检测材料激活补体C3是一种方便可靠的检测手段 ,可作为开发生物材料时对有应用前景的生物材料的筛选和评价。This study was performed to evaluate the degree of complement C3 activation of five biomedical polymers (cellulose acetate(CA), polymethylmethacrylate(PMMA), polysulphone(PS), polyethersulphone(PES), sulphonated polyethersulphone(PES SO 3) which are used to make hemofilters. The concentration of the activation derivative C3a des Arg was measured by radioimmunoassay (RIA) in human plasma after incubation with these five polymers for 30, 60, 90 and 120 minutes. The experimental results indicated that the presence of polymers caused an increase in C3a des Arg. The potential to activate complement C3 decreased in sequence of CA, PMMA, PES, PS and PES SO 3. CA activated the most complement C3. PES SO 3 activated the least complement C3, and the concentration of C3a des Arg decreased with the increase in sulphonation degree. The investigation demonstrates that with good biocompatibility PES is a promising material to make biocompatible hemofilters. Furthermore, it indicates that PES containing sulphonate groups can achieve a marked reduction in complement activation and supports the sulphonation as a means of improving the biocompatibility of PES for blood purification application. RIA is a reliable and precise quantitative determination of human C3a des Arg and is suitable for testing the activation of complement of various synthetic materials that are utilized or to be utilized in the medical field.

关 键 词：血液净化材料 补体激活 过敏毒素C3a-des-Arg 

分 类 号：R318.08[医药卫生—生物医学工程]