人源性抗HBs可变区单链抗体基因的构建及核酸序列测定  

Construction and Nucleotide Sequence Assay of Human Anti-HBs Variable Region Single Chain Antibody Gene

作　　者：任向荣[1] 高辉[2] 粟宽源[1] 陈文吟[1] 杨安钢[2] 余宙耀[1] 

机构地区：[1]中国人民解放军传染病中心医院,广州510602 [2]第四军医大学生化教研室,西安710032

出　　处：《生物医学工程学杂志》2000年第4期484-486,共3页Journal of Biomedical Engineering

摘　　要：将通过噬菌体显示技术获得的一株人源性抗HBsFab抗体基因重链和k轻链的可变区用编码柔性肽段的Linker使其连接成单链 ,克隆入中间载体 ,并进行核酸序列测定 ,为其后的表达及双功能抗体的构建打下基础。The V H and V k gene which were acquired using phage display technology have been combined by a linker encoding a flexible peptide(Gly4 Ser) 3 to form a single chain. Its nucleotide sequence was assayed after cloning the scF v gene into middle plasmid. The successful construction of single chain gene lays the foundations for the subsequent expression and production of double functional antibody.

关 键 词：HBsAg 单链抗体 序列 

分 类 号：R392[医药卫生—免疫学]